POCIS for the monitoring of Glyphosate and AMPA

Glyphosate [(N-phosphonomethyl)glycine] is a non-selective broad-spectrum herbicide that is extensively used in agriculture. The biodegradation of Glyphosate (GLY) in the environment leads to aminomethylphosphonic acid (AMPA).

            Structures of Glyphosate                                     AMPA                                                Glufosinate

        Glyphosate       AMPA Formule                Glufosinate Structure




Conventional sorbents for POCIS do not provide very efficient extractions of these both very polar molecules from water.

A Molecularly Imprinted Polymer (MIP) has been synthesized for the selective extraction of Glyphosate, AMPA and GLUFOSINATE. 

This sorbent can be used for a SPE application (AFFINIMIP® SPE Glyphosate - AMPA) as well as for a POCIS application (AFFINIMIP® POCIS Glyphosate)

Membrane : Polyethersulfone


Posters & Articles

BRGM (French institute of geological survey) has evaluated the passive sampling of 42 pesticides for GROUNDWATER. Two types of passive samplers, a POCIS for polar pesticides, and a POCIS-MIP sampler equivalent to AFFINIMIP® POCIS Glyphosate were studied under low flow conditions as exist in groundwater. Calibrations took place in an experimental pilot filled with groundwater and with few metres water per day. AMPA and glyphosate had similar sampling rates for both tested water-flow values (1 m and 4 m per day), but that were lower than those obtained under stirring conditions by a factor 3 for glyphosate and a factor 7 for AMPA.

Estimating 42 pesticide sampling rates by POCIS and POCIS-MIP samplers for groundwater monitoring: a pilot-scale calibration, C. Berho, S. Robert, C. Coureau, E. Coisy, A. Berrehouc, L. Amalric, A. Bruchet, Environmental Science and Pollution Research (2020) New!


- A polar organic chemical integrative sampler (POCIS) dedicated to glyphosate and its main degradation product aminomethylphosphonic acid (AMPA) have been developped from AFFINIMIP®SPE Glyphosate-AMPA. The laboratory calibration in a continuous water-flow-through exposure system show a sampling rates of 111 and 122 mL day-1 for glyphosate and AMPA respectively. This product is marketed as AFFINIMIP® POCIS GLYPHOSATE.

Laboratory calibration of a POCIS-like sampler based on molecularly imprinted polymers for glyphosate and AMPA sampling in water, C. Berho, B. Claude, E. Coisy, A. Togola, S. Bayoudh, P. Morin, L. Amalric, Anal Bioanal Chem 409: 2029 (2017). 


- New molecularly imprinted polymers (MIP) used as SPE clean up method and as a passive sampler receiving phase for the catchment of glyphosate and AMPA in water, C. Berho, B. Claude, L. Amalric, A. Togola, E. Grellet, S. Bayoudh, K. Puzio, P. Morin with a financial support of the French National Research Agency (ANR):  ECOTECH ORIGAMI PROJECT, Poster presented at SETAC 2015.

A SPE sorbent based on Molecularly Imprinted Polymers (MIP) was developed for Glyphosate and AMPA to be tested as an integrative passive sampler (POCIS-LIKE) receiving phase.


SPE application of this product

- AFFINIMIP® SPE Glyphosate - AMPA was tested on different water matrices (mineral, groundwater, geothermal and sea). The protocol was first applied to mineral water spiked by GLY and AMPA at environmental concentration levels (25-750 ng L−1) using UPLC-MS after derivatization of GLY and AMPA by 9-fluorenylmethylchloroformate. The linear correlation between concentrations measured with and without SPE on MIP was proved. Furthermore, other extractions showed that high concentrations of metal ion interferents (lead(II), cadmium(II), and zinc(II)) in groundwaters did not reduce SPE performance of the MIP. Then, concentration assays were undertaken and brought noteworthy results, such as the recovery of 80% GLY and AMPA from groundwater spiked at 10 ng L−1 and concentrated 100 times. For this purpose, ion exclusion chromatography hyphenated to mass was applied without previous derivatization of the analytes. The same concentration factor and analytical method were applied to 100 ng L−1 spiked sea water with recoveries of 96% for GLY and 121% for AMPA.

Preliminary recovery study of a commercial molecularly imprinted polymer for the extraction of glyphosate and AMPA in different environmental waters using MS, B. Claude, C. Berho, S. Bayoudh, L. Amalric, E. Coisy, R. Nehmé, P. Morin, Environ Sci Pollut Res, 24: 12293 (2017). New!





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