SPE for bioanalysis & proteomics

AttractSPE®Disks 

Due to their unique advantage, AttractSPE®Disks are useful for the purification of very small sample volumes in proteomics, genomics, metabolomics, biomarker discovery and biological applications. They are applied for spinnable, automatable, high-throughput microextraction and nanoextraction.

AttractSPE®Disks offer exceptional efficiency in sample preparation and reproducibility of results. Since the diffusion distance between the particles is minimised, adsorption is more efficient and extraction can be achieved using a very low sorbent mass.

These properties give AttractSPE®Disks a significant improvement in mass transfer kinetics compared to conventional packed SPE particles. Being a monolithic disc, AttractSPE®Disks are self-sufficient and do not require frits to immobilize the column bed (unlike traditional SPE products) allowing recovery of 100% of the original sample volume.

 

gammes produits microextractions bandeau bleu opaque avec noms

Advantages of SPE disksÔÇő :

  • No need to frits or filters
  • Reduced dead volume
  • Small elution volumes
  • High sample recovery
  • Reduced time for eluate evaporation
  • Higher throughput
  • Channeling effects eliminated
  • Excellent reproducibility
  • Concentration of the sample

Format microextraction

AttractSPE®Disks can be used to miniaturized SPE and due to the small operating volume of fluid samples available. So there are available under 4 formats for microextraction.

AttractSPE®Disks Tips – Stagetips

µSPE column designed by immobilizing an uniform disk inside a pipette tips (Stagetips)

tips

 

 

 

 

 

 

 

AttractSPE®Disks Spin SPE

SPE column designed by immobilizing an uniform disk inside a centrifuge SPE microtube

Copie de ┬Áspin

 

 

 

 

 

 

 

AttractSPE®Disks 96 well-plate SPE

96 SPE wellplate column designed by immobilizing an uniform disk

Copie de 96plate tip 1

 

 

 

 

 

 

AttractSPE®Disks SPE cartridges

SPE column designed by immobilizing an uniform disk

ATTRACTspe DISKS CARTRIDGES

 

 

 

 

 

 

 

Visit our catalog of clean-up tools for biomolecular applications

 

SorbentsÔÇő

• A broad variety of sorbents for each required applications

• Various formats: disks, spins, 96 SPE plates, cartridges

• One sorbent - several thicknesses for increased capacity

• Several sorbents - stacking for complex applications

• Disks used as filter for application requiring beads

 

Sorbents for SPE Disks for biomolecular applications

Compatible with analytical methods

C18

- Desalting of peptides; fractionation of peptides at acidic and neutral pH

- Drug extraction in biological samples

  C8

  Desalting of large peptides and proteins; Usage as frits to retain beads in a tip

Silica

Purification of DNA

C4

Desalting of large peptides and proteins

SDB a.k.a PS-DVB

Fractionation of peptides at basic pH

HLB: SDB with hydrophilic moieties  ÔÇő

Fractionation of peptides Extraction of small molecules (drugs) in biological fluidsÔÇő

SDB – RPS: Sulfonic modified SDB sorbent  ÔÇő

Desalting of peptides ; Fractionation of peptides

SAX : Anion exchange SDB 

Fractionation of peptides by salt or pH steps

SCX: Cation exchange SDB 

Fractionation of peptides by salt or pH steps

 

Capacity

Each product is available with different capacities, which are designated T1, T2 or T3 in the product designations. This designation refers to the layer thickness of SPE tips. For a more reliable product, each tool contains only ONE layer.

Tips T1 T2 T3

- T1 : capacity up to 15µg

- T2 : capacity up to 30µg

- T3 : capacity up to 50µg

 

 

 

Articles :

- Collagen peptide mass fingerprinting, best known as Zooarchaeology by Mass Spectrometry (or ZooMS) when applied to archaeology, has become invaluable for the taxonomic identification of archaeological collagenous materials, in particular fragmentary and modified bone remains. Prior to MALDI-based spectrometric analysis, collagen needs to be extracted from the bone's inorganic matrix, isolated and purified.

Archaeological samples were purified according to SP3 (Single-Pot, Solid-Phase, Sample-Preperation)method using StageTips (equivalent to AttractSPE®Disks Tips C18 ref C18.T2.200.96) from two trimmed AttractSPE® Disks Bio C18 (SPE-Disks-Bio-C18-100.47.20).

Testing the efficacy and comparability of ZooMS protocols on archaeological bone, Wang Naihui, Brown Samantha, Ditchfield Peter, Hebestreit Sandra, Kozilikin Maxim, Luu Sindy, Wedage Oshan, Grimaldi Stefano, Chazan Michael, Horwitz Kolska Liora, Spriggs Matthew, Summerhayes Glenn, Shunkov Michael, Richter Korzow Kristine, Douka Katerina, Journal of Proteomics, Volume 233, 104078, 2021, https://doi.org/10.1016/j.jprot.2020.104078 - New !

 

- Pre-mRNA splicing catalyzed by the spliceosome represents a critical step in the regulation of gene expression contributing to transcriptome and proteome diversity. In this papers, researchers from several institutes of University of Cologne (Germany) have studied the role of Ecdysoneless (Ecd) as a critical regulator of U5 snRNP assembly and Prp8 stability.

During there experiments, protein identification prior to mass spectrometry, AttractSPE®Disks Bio SDB-RPS (SPE-Disk-Bio-RPS-47.20) have been used as Stagetips.

Ecd promotes U5 snRNP maturation and Prp8 stability, Steffen Erkelenz, Dimitrije Stankovi─ç, Juliane Mundorf, Tina Bresser, Ann-Katrin Claudius, Volker Boehm, Niels H Gehring, Mirka Uhlirova, Nucleic Acids Research, 2021;, gkaa1274, https://doi.org/10.1093/nar/gkaa1274 Open access - New !

 

- In this article, proximity labeling technique optimized for studying proteins in yeast mitochondria is described. Importantly, BioID (biotin identification) permits the identification of transiently interacting proteins and also proteins located in vicinity of the bait, which may not be co-purified by conventional pull down experiments. Stagetips based on AttractSPE®Disks Bio SDB-RPS (SPE-Disks-Bio-RPS-47.20) or commercially available (Tips-RPS-M.T2.200.96) were used to purify bionylated proteins prior to Mass spectroscopy analysis.

Mapping protein networks in yeast mitochondria using proximity-dependent biotin identification coupled to proteomics, Roger Salvatori, Wasim Aftab, Ignasi Forne, Axel Imhof, Martin Ott, Abeer Prakash Singh,  STAR Protocols, Volume 1, Issue 3, 2020, 100219, https://doi.org/10.1016/j.xpro.2020.100219 OPEN ACCESS - New !

 

- In this article, high-throughput genetic screens were performed that provide a novel global map of histone residues required for transcriptional reprogramming in response to heat and osmotic stress.

In this study, fractionation of phosphopeptides at high pH was performed using gel loading tips packed with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and containing 1 mg C18 material.

A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress, Cristina Viéitez, Gerard Martínez-Cebrián, Carme Solé, René Bö ttcher, Clement M Potel, Mikhail M Savitski, Sara Onnebo, Marc Fabregat, Ali Shilatifard, Francesc Posas, Eul`lia de Nadal, Nucleic Acids Research, Band 48, Ausgabe 7, 17. April 2020, Seiten 3455–3475, https://doi.org/10.1093/nar/gkaa081 OPEN ACCESS - New !

 

- The combination of thermal proteome profiling (TPP) with phosphoproteomics (phospho-TPP) offers a way to assess the functional relevance of identified phosphorylation sites on a proteome-wide scale by comparing the melting behaviour of a protein and its phosphorylated form(s). In this article, phospho-TPP experiments were performed in HeLa cells using an optimised protocol.

In this study, phosphopeptide fractionation was performed with gel loading tips plugged with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and packed with 1 mg C18 material.

Impact of phosphorylation on thermal stability of proteins, Clément M. Potel, Nils Kurzawa, Isabelle Becher, Athanasios Typas, André Mateus, Mikhail M. Savitski

bioRxiv 2020.01.14.903849; doi: https://doi.org/10.1101/2020.01.14.903849 Open access - New !

 

Application noteÔÇő

This application note compares the performance of AttractSPE®Disks Tips C18 and two marketed SPE Tips C18 for peptide purification. As a model, standard protein (BSA, Bovine Serum Albumin) was digested with trypsin and the peptides were then desalted and concentrated using SPE Tips C18. The peptides were finally analysed by nanoLC-MS/MS. The data generated show excellent performance of AttractSPE®Disks Tips C18, which is similar or even better than the two marketed SPE tips and allows easy and reliable protein identification.

Learn more :

AN AttractSPE­­®Disks Tips C18 - Peptides

 

Related products

As your need for stagetips experiments grows, AttractSPE® Plate 96 for Microelution is THE format to perform 96 experiments in one step through automation. Weitere Informationen finden Sie hier

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Do not hesitate to contact us for any format or additional information

 

attractspe disks environment

AttractSPE®Disks BioMol

SPE Disks C18, SAX, SCX, RPS for Bioanalytical Applications, Peptide Purification, Desalting Proteins...


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