SPE for bioanalysis & proteomics

AttractSPE®Disks are thin, dense, soft and uniform membranes based chromatography for extraction/separation, purification and concentration of analyte molecules.

Thanks to their unique advantage, AttractSPE®Disks are useful for purification of Very Small Sample Volumes in Proteomics, Genomics, Metabolomics, Biomarker discoveries and Biological applications. They are applied for Spinnable, Automatable, High throughput microextraction and nano extraction.

AttractSPE®Disks offer outstanding sample preparation efficiency and reproducibility of results. Since the diffusion distance between particles is minimized, adsorption is more efficient, and extraction can be accomplished using very low sorbent mass.

These properties are giving to AttractSPE®Disks a significant improvement of mass transfer kinetics compared to traditional packed SPE particles. As a monolith disk, AttractSPE®Disks are self stand and require no frits for immobilizing the column bed (unlike traditional SPE products) allowing 100% recovery of the original sample volume.

gammes produits microextractions bandeau bleu opaque avec noms

Advantages of SPE disks​ :

  • No need to frits or filters
  • Reduced dead volume
  • Small elution volumes
  • High sample recovery
  • Reduced time for eluate evaporation
  • Higher throughput
  • Channeling effects eliminated
  • Excellent reproducibility
  • Concentration of the sample

Format microextraction

AttractSPE®Disks can be used to miniaturized SPE and due to the small operating volume of fluid samples available. So there are available under 4 formats for microextraction.

AttractSPE®Disks Tips – Stagetips

µSPE column designed by immobilizing an uniform disk inside a pipette tips (Stagetips)

tips

 

 

 

 

 

 

 

AttractSPE®Disks Spin SPE

SPE column designed by immobilizing an uniform disk inside a centrifuge SPE microtube

Copie de µspin

 

 

 

 

 

 

 

AttractSPE®Disks 96 well-plate SPE

96 SPE wellplate column designed by immobilizing an uniform disk

Copie de 96plate tip 1

 

 

 

 

 

 

AttractSPE®Disks SPE cartridges

SPE column designed by immobilizing an uniform disk

ATTRACTspe DISKS CARTRIDGES

 

 

 

 

 

 

 

Visit our catalog of clean-up tools for biomolecular applications

 

Sorbents​

• A broad variety of sorbents for each required applications

• Various formats: disks, spins, 96 SPE plates, cartridges

• One sorbent - several layers for increased capacity

• Several sorbents - stacking for complex applications

• Disks used as filter for application requiring beads

 

Sorbents for SPE Disks for biomolecular applications

Compatible with analytical methods

C18

- Desalting of peptides; fractionation of peptides at acidic and neutral Ph

- Drug extraction in biological samples

  C8

  Desalting of large peptides and proteins; Usage as frits to retain beads in a tip

Silica

Purification of DNA

C4

Desalting of large peptides and proteins

SDB a.k.a PS-DVB

Fractionation of peptides at basic pH

HLB: SDB with hydrophilic moieties  ​

Fractionation of peptides Extraction of small molecules (drugs) in biological fluids​

SDB – RPS: Sulfonic modified SDB sorbent  ​

Desalting of peptides ; Fractionation of peptides

SAX : Anion exchange SDB 

Fractionation of peptides by salt or pH steps

SCX: Cation exchange SDB 

Fractionation of peptides by salt or pH steps

Articles :

- High-throughput genetic screenings that provide a novel global map of the histone residues required for transcriptional reprogramming in response to heat and osmotic stress was performed in this article.

In this study, phosphopeptides high pH fractionation was carried out using gel loader tips plugged with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and packed with 1 mg of C18 material.

 

A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress, Cristina Viéitez, Gerard Martínez-Cebrián, Carme Solé, René Böttcher, Clement M Potel, Mikhail M Savitski, Sara Onnebo, Marc Fabregat, Ali Shilatifard, Francesc Posas, Eulàlia de Nadal, Nucleic Acids Research, Volume 48, Issue 7, 17 April 2020, Pages 3455–3475, https://doi.org/10.1093/nar/gkaa081 Open access - New !

 

- Combining thermal proteome profiling (TPP) with phosphoproteomics (phospho-TPP) provides a way to assess the functional relevance of identified phosphorylation sites on a proteome-wide scale by comparing the melting behavior of a protein and its phosphorylated form(s). In this article, phospho-TPP experiments in HeLa cells was performed with an optimized protocol.

In this study, phosphopeptides fractionation was carried out using gel loader tips plugged with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and packed with 1 mg of C18 material.

 

Impact of phosphorylation on thermal stability of proteins, Clément M. Potel, Nils Kurzawa, Isabelle Becher, Athanasios Typas, André Mateus, Mikhail M. Savitski

bioRxiv 2020.01.14.903849; doi: https://doi.org/10.1101/2020.01.14.903849 Open access - New !

 

Application Notes​

In this application note, performances of AttractSPE®Disks Tips C18 and two marketed SPE tips C18 are compared for the peptidic purification. As a model, the standard protein (BSA, Bovin Serum Albumin) was digested with trypsin and the peptides were then desalted and concentrated with SPE Tips C18. Peptides were finally analysed by nanoLC-MS/MS. Generated data show excellent performance of the AttractSPE®Disks Tips C18 similar or even better than both marketed SPE tips and make possible an easy and reliable identification of the proteins.

Learn more :

AN Application Note AttractSPE®Disks Tips C18 - Peptides

 

attractspe disks environment

AttractSPE®Disks BioMol

SPE Disks C18, SAX, SCX, RPS for Bioanalytical Applications, Peptide Purification, Desalting Proteins...


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