AttractSPE®Disks BioMol

SPE Disks C18, SAX, SCX, RPS for Bioanalytical Applications, Peptide Purification, Desalting Proteins...

As protein analysis has become more complex and more sensitive, the need for adequate materials meets more specific requirements. Proteomic sample preparation should be a critical consideration.

AFFINISEP brings to you a full range of membranes with AttractSPE®Disks BioMol with C18, C8, C4, SCX, SAX, RPS.... This SPE membranes are especially designed and made by AFFINISEP for your bioanalytical needs and to help you to develop your own tools for your R&D projects, for instance to make Stagetips by punching the membranes. Our high quality SPE membranes allow high throughput (HTS) for protein desalting or peptides purification.

AttractSPE®Disks BioMol are fully compatible with automated liquid handlers. Indeed, with these Solid Phase Extraction Disks, you can design the applications you wish such as removing impurities from samples and isolation of analytes from biomatrices with small elution volumes. Fast and efficient purification of peptides through Solid Phase Extraction is easily accessible.

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Product Compatible with analytical methods
AttractSPE®Disks Bio - C18

SPE Disks  with C18 sorbent for biomolecular applications

Desalting of peptides; fractionation of peptides at acidic and neutral pH

AttractSPE®Disks Bio - C8

SPE Disks  with C8 sorbent for biomolecular applications

Desalting of large peptides and proteins; Usage as frits to retain beads in a tip

AttractSPE®Disks Bio - C4

SPE Disks  with C4 sorbent for biomolecular applications

Desalting of large peptides and proteins

AttractSPE®Disks Bio - SDB

SPE Disks with PS-DVB sorbent for biomolecular applications

Fractionation of peptides at basic pH

AttractSPE®Disks Bio - HLB

SPE Disks of DVB with hydrophilic moieties (HLB) sorbent for biomolecular applications

Fractionation of peptides

AttractSPE®Disks Bio SDB - RPS

SPE Disks with sulfonic modified PS-DVB sorbent for biomolecular applications

A Styrenedivinylbenzene resin modified with sulfonic acid groups

Desalting of peptides; fractionation of peptides

AttractSPE®Disks Bio - SAX for biomolecular applications

Fractionation of peptides by salt or pH steps

AttractSPE®Disks Bio - SCX for biomolecular applications

Fractionation of peptides by salt or pH steps

Product references :

Product 25mm, 40/pk 47mm, 20/pk
AttractSPE®Disks Bio - HLB

SPE Disks of DVB with hydrophilic moieties (HLB) sorbent for biomolecular applications

SPE-Disks-Bio-HLB-25.40 SPE-Disks-Bio-HLB-47.20
AttractSPE®Disks Bio -  C18

SPE Disks  with C18 sorbents for biomolecular applications

SPE-Disks-Bio-C18-100.25.40 SPE-Disks-Bio-C18-100.47.20
AttractSPE®Disks Bio - C8

SPE Disks  with C8 sorbents for biomolecular applications

SPE-Disks-Bio-C8-100-25.40 SPE-Disks-Bio-C8-100.47.20
AttractSPE®Disks Bio - C4

SPE Disks  with C4 sorbents for biomolecular applications

SPE-Disks-Bio-C4.300-25.40 SPE-Disks-Bio-C4.300-47.20
AttractSPE®Disks Bio - SDB

SPE Disks with PS-DVB sorbent for biomolecular applications

SPE-Disks-Bio-DVB-25.40 SPE-Disks-Bio-DVB-47.20
AttractSPE®Disks Bio - SDB-RPS

SPE Disks with sulfonic modified PS-DVB sorbent for biomolecular applications

SPE-Disks-Bio-RPS-25.40

SPE-Disks-Bio-RPS-47.20

AttractSPE®Disks Bio - SAX

SPE Disks with SAX sorbent for biomolecular applications

SPE-Disks-Bio-SAX-25.40 SPE-Disks-Bio-SAX-47.20
AttractSPE®Disks Bio - SCX

SPE Disks with SCX sorbent for biomolecular applications

SPE-Disks-Bio-SCX-25.40 SPE-Disks-Bio-SCX-47.20

 

Articles :

- High-throughput genetic screenings that provide a novel global map of the histone residues required for transcriptional reprogramming in response to heat and osmotic stress was performed in this article.

In this study, phosphopeptides high pH fractionation was carried out using gel loader tips plugged with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and packed with 1 mg of C18 material.

 

A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress, Cristina Viéitez, Gerard Martínez-Cebrián, Carme Solé, René Böttcher, Clement M Potel, Mikhail M Savitski, Sara Onnebo, Marc Fabregat, Ali Shilatifard, Francesc Posas, Eulàlia de Nadal, Nucleic Acids Research, Volume 48, Issue 7, 17 April 2020, Pages 3455–3475, https://doi.org/10.1093/nar/gkaa081 Open access - New !

 

- Combining thermal proteome profiling (TPP) with phosphoproteomics (phospho-TPP) provides a way to assess the functional relevance of identified phosphorylation sites on a proteome-wide scale by comparing the melting behavior of a protein and its phosphorylated form(s). In this article, phospho-TPP experiments in HeLa cells was performed with an optimized protocol.

In this study, phosphopeptides fractionation was carried out using gel loader tips plugged with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and packed with 1 mg of C18 material.

 

Impact of phosphorylation on thermal stability of proteins, Clément M. Potel, Nils Kurzawa, Isabelle Becher, Athanasios Typas, André Mateus, Mikhail M. Savitski

bioRxiv 2020.01.14.903849; doi: https://doi.org/10.1101/2020.01.14.903849 Open access - New !

 

 

AttractSPE®Disks Bio - C18 - 25mm - 40/pk

Reference : SPE-Disks-Bio-C18-100.25.40
For more information

AttractSPE®Disks Bio - C18 - 47mm - 20/pk

Reference : SPE-Disks-Bio-C18-100.47.20
For more information

AttractSPE®Disks Bio - C4 - 25mm - 40/pk

Reference : SPE-Disks-Bio-C4-300.25.40
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AttractSPE®Disks Bio - C4 - 47mm - 20/pk

Reference : SPE-Disks-Bio-C4-300.47.20
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AttractSPE®Disks Bio - C8 - 25mm - 40/pk

Reference : SPE-Disks-Bio-C8-100.25.40
For more information

AttractSPE®Disks Bio - C8 - 47mm - 20/pk

Reference : SPE-Disks-Bio-C8-100.47.20
For more information


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