AttractSPE®Disks Tips – StageTips

AttractSPE®Tips are centrifugeable SPE tips, compatible with automates for high throughput screening useful for biomolecular applications of peptide desalting, proteomics, and biomarker discovery.

In order to prepare high quality peptide samples for LC-MS analysis, it is very important to ensure excellent overall quality of proteomic experiments. Peptide samples collected after enzymatic digestion require treatment to remove salts, possible pieces of gels or particles that can damage the equipment or falsify the results.

 

AttractSPE®Tips is the ideal tool for this application as well as for drug extraction or small molecule analysis in urine or serum in bioanalysis.


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Capacity

Each product is available with different capacities, which are designated T1, T2 or T3 in the product designations. This designation refers to the layer thickness of SPE tips. For a more reliable product, each tool contains only ONE layer.

Tips T1 T2 T3

- T1 : capacity up to 15µg

- T2 : capacity up to 30µg

- T3 : capacity up to 50µg

 

 

 

Product references

For the sake of clarity, the references below refer only to T1 capacity. If you want T2 or T3 capacity, modify T1 in the reference by the capacity you are interested in.

Designation

Description

Reference – 200µl - 96/pk

Reference –1mL - 96/pk

AttractSPE®Tips C18

Membrane C18 , 96/pk

Tips-C18.T1.200.96

Tips-C18.T1.1000.96

AttractSPE®Tips C8

Membrane C8, 96/pk

Tips-C8.T1.200.96

Tips-C8.T1. 1000.96

AttractSPE® Disks Tips HLB

HLB membrane, 96/pk

Tips-HLB.T1.200.96

Tips-HLB.T1. 1000.96

AttractSPE®Tips SDB

Membrane PS-DVB, 96/pk

Tips-DVB.T1.200.96

Tips-DVB.T1. 1000.96

AttractSPE®Tips  SDB - RPS

Membrane DVB modifiée, 96/pk

Tips-RPS.T1.200.96

Tips-RPS.T1. 1000.96

AttractSPE®Tips SAX

Membrane SAX, 96/pk

Tips-SAX.T1.200.96

Tips-SAX.T1. 1000.96

AttractSPE®Tips SCX

Membrane SCX, 96/pk

Tips-SCX.T1.200.96

Tips-SCX.T1. 1000.96

AttractSPE®Tips C18-SCX

Membrane multicouche C18 & SCX, 96/pk

Tips-C18-SCX.T1.200.96

Tips-C18-SCX.T1. 1000.96

AttractSPE®Tips C18-SCX-C18

Membrane multicouche C18 & SCX &C18, 96/pk

Tips-C18-SCX-C18.T1.200.96

Tips-C18-SCX-C18.T1. 1000.96

AttractSPE®Tips SDB-SAX

Membrane multicouche PS-DVB & SAX, 96/pk

Tips-DVB-SAX.T1.200.96

Tips-DVB-SAX.T1. 1000.96

AttractSPE®Tips Silica

Membrane Silica, 96/pk

Tips-Si.T1.200.96

Tips-Si.T1. 1000.96

Tips Volumes : 200, 300, 500, 1000 µL

 

Articles :

- Collagen peptide mass fingerprinting, best known as Zooarchaeology by Mass Spectrometry (or ZooMS) when applied to archaeology, has become invaluable for the taxonomic identification of archaeological collagenous materials, in particular fragmentary and modified bone remains. Prior to MALDI-based spectrometric analysis, collagen needs to be extracted from the bone's inorganic matrix, isolated and purified.

Archaeological samples were purified according to SP3 (Single-Pot, Solid-Phase, Sample-Preperation)method using StageTips (equivalent to AttractSPE®Disks Tips C18 ref C18.T2.200.96) from two trimmed AttractSPE® Disks Bio C18 (SPE-Disks-Bio-C18-100.47.20).

Testing the efficacy and comparability of ZooMS protocols on archaeological bone, Wang Naihui, Brown Samantha, Ditchfield Peter, Hebestreit Sandra, Kozilikin Maxim, Luu Sindy, Wedage Oshan, Grimaldi Stefano, Chazan Michael, Horwitz Kolska Liora, Spriggs Matthew, Summerhayes Glenn, Shunkov Michael, Richter Korzow Kristine, Douka Katerina, Journal of Proteomics, Volume 233, 104078, 2021, https://doi.org/10.1016/j.jprot.2020.104078 - New !

 

- Pre-mRNA splicing catalyzed by the spliceosome represents a critical step in the regulation of gene expression contributing to transcriptome and proteome diversity. In this papers, researchers from several institutes of University of Cologne (Germany) have studied the role of Ecdysoneless (Ecd) as a critical regulator of U5 snRNP assembly and Prp8 stability.

During there experiments, protein identification prior to mass spectrometry, AttractSPE®Disks Bio SDB-RPS (SPE-Disk-Bio-RPS-47.20) have been used as Stagetips.

Ecd promotes U5 snRNP maturation and Prp8 stability, Steffen Erkelenz, Dimitrije Stanković, Juliane Mundorf, Tina Bresser, Ann-Katrin Claudius, Volker Boehm, Niels H Gehring, Mirka Uhlirova, Nucleic Acids Research, 2021;, gkaa1274, https://doi.org/10.1093/nar/gkaa1274 Open access - New !

 

- In this article, proximity labeling technique optimized for studying proteins in yeast mitochondria is described. Importantly, BioID (biotin identification) permits the identification of transiently interacting proteins and also proteins located in vicinity of the bait, which may not be co-purified by conventional pull down experiments. Stagetips based on AttractSPE®Disks Bio SDB-RPS (SPE-Disks-Bio-RPS-47.20) or commercially available (Tips-RPS-M.T2.200.96) were used to purify bionylated proteins prior to Mass spectroscopy analysis.

Mapping protein networks in yeast mitochondria using proximity-dependent biotin identification coupled to proteomics, Roger Salvatori, Wasim Aftab, Ignasi Forne, Axel Imhof, Martin Ott, Abeer Prakash Singh,  STAR Protocols, Volume 1, Issue 3, 2020, 100219, https://doi.org/10.1016/j.xpro.2020.100219 OPEN ACCESS - New !

 

- In this article, high-throughput genetic screens were performed that provide a novel global map of histone residues required for transcriptional reprogramming in response to heat and osmotic stress.

In this study, fractionation of phosphopeptides at high pH was performed using gel loading tips packed with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and containing 1 mg C18 material.

A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress, Cristina Viéitez, Gerard Martínez-Cebrián, Carme Solé, René Bö ttcher, Clement M Potel, Mikhail M Savitski, Sara Onnebo, Marc Fabregat, Ali Shilatifard, Francesc Posas, Eul`lia de Nadal, Nucleic Acids Research, Band 48, Ausgabe 7, 17. April 2020, Seiten 3455–3475, https://doi.org/10.1093/nar/gkaa081 OPEN ACCESS - New !

 

- The combination of thermal proteome profiling (TPP) with phosphoproteomics (phospho-TPP) offers a way to assess the functional relevance of identified phosphorylation sites on a proteome-wide scale by comparing the melting behaviour of a protein and its phosphorylated form(s). In this article, phospho-TPP experiments were performed in HeLa cells using an optimised protocol.

In this study, phosphopeptide fractionation was performed with gel loading tips plugged with AttractSPE®Disks C18 (equivalent to our AttractSPE® Disks Tips C18 and packed with 1 mg C18 material.

Impact of phosphorylation on thermal stability of proteins, Clément M. Potel, Nils Kurzawa, Isabelle Becher, Athanasios Typas, André Mateus, Mikhail M. Savitski

bioRxiv 2020.01.14.903849; doi: https://doi.org/10.1101/2020.01.14.903849 Open access - New !

 

Application note

This application note compares the performance of AttractSPE®Disks Tips C18 and two marketed SPE Tips C18 for peptide purification. As a model, standard protein (BSA, Bovine Serum Albumin) was digested with trypsin and the peptides were then desalted and concentrated using SPE Tips C18. The peptides were finally analysed by nanoLC-MS/MS. The data generated show excellent performance of AttractSPE®Disks Tips C18, which is similar or even better than the two marketed SPE tips and allows easy and reliable protein identification.

Learn more :

AN AttractSPE­­®Disks Tips C18 - Peptides

 

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