AFFINIMIP® SPE Chloramphenicol

Specific extraction of Chloramphenicol from products of animal origin and biological matrices

Chloramphenicol: a major concern for human health and a challenge in food safety analysis

Chloramphenicol is a broad-spectrum antibiotic widely used in the world in the past. Several health problems are related to its use. As a consequence, several countries (e.g. U.S.A, E.U, Canada...) have prohibited its use for food-producing animals. However, due to its broad spectrum of activity and its availability, Chloramphenicol is still used in several countries to treat food-producing animals. Therefore, chloramphenicol analysis is still a current affair. As no permitted limit has been established, E.U. has defined a Minimum Required Performance Limit (MRPL) set at 0.3µg/kg for products of animal origin (Commission decision 2003/181/EC).

In addition, food matrices are very complexes and induce ion-suppression phenomena which distort analysis results. For such a low MRPL threshold, a cleanup step is crucial in order to improve the sensitivity, the reliability and the specificity before analysis. It is therefore critical to develop a highly selective and sensitive analytical assay to control and monitor Chloramphenicol residues in difficult matrices such as food stuffs.

 

How to solve this?

AFFINISEP has developed a new class of intelligent polymers (AFFINIMIP®) based on Molecularly Imprinted Polymers specific to Chloramphenicol used as a powerful technique for sample cleanup and pre-concentration. AFFINIMIP® SPE Chloramphenicol cartridge is a simple, fast, sensitive and selective tool for the extraction of Chloramphenicol from complex matrices.

 

Benefits

  • Ready for use and optimized extraction protocol
  • Robust and Fast SPE protocol (around 30min) 
  • No use of halogenated solvent
  • Perfect clean-up system for trace analysis of Chloramphenicol
  • Enrichment of the analyte
  • Strong reduction of interferents and matrix effects
  • No Liquid - Liquid Extraction
  • Excellent cost-effective products

Main format available

Designation, Format of cartridges, amount of sorbent

Box composition

Reference

AFFINIMIP® SPE Chloramphenicol

Open cartridge 1mL 

25 Cartridges

FS110-02A

50 Cartridges

FS110-03A

AFFINIMIP® SPE Chloramphenicol

Open cartridge 3mL 

25 Cartridges

FS110-02

50 Cartridges

FS110-03

Other formats can be supplied on demand

Application notes

Milk matrices

This study describes the solid phase extraction of Chloramphenicol from Milk at 22ng/mL. We demonstrate in this application note that a reliable quantification of Chloramphenicol at low concentrations and single quadrupole mass spectrometry detection is possible using AFFINIMIP® SPE Chloramphenicol (1mL or 3mL). The results demonstrate the efficiency of the clean-up: high recovery yield (> 85%) and a good repeatability.

Application note: Selective Solid Phase Extraction for Chloramphenicol Analysis from Milk using AFFINIMIP® SPE

Honey matrices

This study describes the solid phase extraction of Chloramphenicol from Honey at Minimum Required Performance Limits (MRPLs) of 0.3µg/kg as defined by European Commission decision 2003/181/EC. We demonstrate in this application note that a reliable quantification of Chloramphenicol at low concentrations using AFFINIMIP® SPE Chloramphenicol and single quadrupole mass spectrometry detection is possible. The results demonstrate the efficiency of the clean-up: high recovery yield (> 90%), a good repeatability and reproducibility were obtained.

Application note: Selective Solid Phase Extraction for Chloramphenicol Analysis from Honey using AFFINIMIP® SPE

Bovine urine

This study demonstrates a reliable quantification of Chloramphenicol from bovine urine (recoveries >90%) using AFFINIMIP® SPE Chloramphenicol and even a single quadrupole mass detection.

Application note AFFINIMIP® SPE Chloramphenicol from bovine urine

Shrimp

We demonstrate in this application note that a reliable quantification of Chloramphenicol from shrimp (recoveries>95%) using AFFINIMIP® SPE Chloramphenicol and a single quadrupole mass detection is possible. UV Chromatograms demonstrate as well a perfect cleanup and a very low background at the retention time of Chloramphenicol.

Application note: Selective Solid Phase Extraction of Chloramphenicol from shrimp using AFFINIMIP® SPE

 

 


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