Extraction of Glyphosate and AMPA

Glyphosate [(N-phosphonomethyl)glycine] is a non-selective broad-spectrum herbicide that is extensively used in agriculture. The biodegradation of Glyphosate (GLY) in the environment leads to aminomethylphosphonic acid (AMPA) as well as glufosinate.

Conventional SPE sorbents do not provide very efficient extractions of these very polar molecules.

AFFINIMIP®SPE Glyphosate - AMPA are selective solid phase extraction cartridges that selectively clean and concentrate Glyphosate, AMPA and glufosinate prior to analysis by HPLC without derivatization.

This product is also available for passive sampling format (AFFINIMIP® POCIS GLYPHOSATE).

Tested matrices: water (surface, river, underground, geothermal), cereals, urine, honey, tea, ...

            Structures of Glyphosate                                     AMPA                                                Glufosinate

        Glyphosate       AMPA Formule                Glufosinate Structure


  • Ready for use and optimized extraction protocol for several matrices (Water, Tea, Cereals, Honey...)
  • Perfect clean-up system for trace analysis of Glyphosate, AMPA, Glufosinate and some metabolites
  • Enrichment of these analytes
  • High recovery yields
  • NO Derivatization required for LC-MS/MS analysis

Booklet AFFINIMIP®SPE Glyphosate New!

Our Booklet AFFINIMIP®SPE Glyphosate collects main useful information about :

  • AFFINIMIP®SPE Glyphosate description
  • Application notes for several complex matrices
  • Tested with several analytical methods with or without derivatization
  • Analytical conditions and transitions for LC-MS/MS analysis without derivatization
  • Passive samplers application for glyphosate - AMPA
  • Related products (accessories)

Main available formats

Designation, Format of cartridges, amount of sorbent Box composition Reference
AFFINIMIP® SPE Glyphosate - AMPA - 6mL cartridge

25 cartridges


50 cartridges

AFFINIMIP® SPE Glyphosate - AMPA - 12mL cartridge

25 cartridges


50 cartridges


Application notes

Determination of Glyphosate, AMPA and GLUFOSINATE in 100mL of water WITHOUT derivatization using AFFINIMIP®SPE Glyphosate

AFFINIMIP®SPE Glyphosate - AMPA - Glufosinate - Water - no derivatization

And more applications are described in AFFINIMIP®SPE Glyphosate application note booklet with main matrices tested (Cereals, Tea, Juice, Honey, water...)

Posters & Articles

- AFFINIMIP® SPE Glyphosate was tested on different water matrices (mineral, groundwater, geothermal and sea). The protocol was first applied to mineral water spiked by GLY and AMPA at environmental concentration levels (25-750 ng L−1) using UPLC-MS after derivatization of GLY and AMPA by 9-fluorenylmethylchloroformate. The linear correlation between concentrations measured with and without SPE on MIP was proved. Furthermore, other extractions showed that high concentrations of metal ion interferents (lead(II), cadmium(II), and zinc(II)) in groundwaters did not reduce SPE performance of the MIP. Then, concentration assays were undertaken and brought noteworthy results, such as the recovery of 80% GLY and AMPA from groundwater spiked at 10 ng L−1 and concentrated 100 times. For this purpose, ion exclusion chromatography hyphenated to mass was applied without previous derivatization of the analytes. The same concentration factor and analytical method were applied to 100 ng L−1 spiked sea water with recoveries of 96% for GLY and 121% for AMPA.

Preliminary recovery study of a commercial molecularly imprinted polymer for the extraction of glyphosate and AMPA in different environmental waters using MS, B. Claude, C. Berho, S. Bayoudh, L. Amalric, E. Coisy, R. Nehmé, P. Morin, Environ Sci Pollut Res, 24: 12293 (2017). New!

- New molecularly imprinted polymers (MIP) used as SPE clean up method and as a passive sampler receiving phase for the catchment of glyphosate and AMPA in water, C. Berho, B. Claude, L. Amalric, A. Togola, E. Grellet, S. Bayoudh, K. Puzio, P. Morin with a financial support of the French National Research Agency (ANR):  ECOTECH ORIGAMI PROJECT, Poster presented at SETAC 2015.

A SPE sorbent based on Molecularly Imprinted Polymers (MIP) was developed for Glyphosate and AMPA to be tested as an integrative passive sampler (POCIS-LIKE) receiving phase.

POCIS application of this product

- A polar organic chemical integrative sampler (POCIS) dedicated to glyphosate and its main degradation product aminomethylphosphonic acid (AMPA) have been developped from AFFINIMIP®SPE Glyphosate. The laboratory calibration in a continuous water-flow-through exposure system show a sampling rates of 111 and 122 mL day-1 for glyphosate and AMPA respectively. This product is marketed as AFFINIMIP® POCIS GLYPHOSATE.

Laboratory calibration of a POCIS-like sampler based on molecularly imprinted polymers for glyphosate and AMPA sampling in water, C. Berho, B. Claude, E. Coisy, A. Togola, S. Bayoudh, P. Morin, L. Amalric, Anal Bioanal Chem 409: 2029 (2017).




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