AFFINIMIP® SPE - Selectives MIP SPE Cartridges

AFFINIMIP® SPE are high performance Solid Phase Extraction sorbents based on molecularly imprinted polymers (MIP) for sample cleanup before chromatographic analytical methods. Thanks to the superior selectivity in comparison with standard SPE, analytical chemists obtain higher recovery yields using simple and ready-to-use protocol.

Download our Sample Preparation catalog (contact us to receive a paper version)

Download our application notebook which gives an overview of application notes (contact us to receive a paper version).

AFFINISEP designs a highly cross-linked polymer to specifically recognize one target compound (compound specific) or structurally related target compounds (class-selective), based on the shape and chemical functions using an imprinting procedure. Several formats are available.

Perfect sample cleanup and lower detection limits

Chromatograms of apple puree containing 40µg/kg or 80µg/kg of Patulin before (Red) and after (Blue) AFFINIMIP® SPE Patulin Clean-up

Based on molecularly imprinted polymers, AFFINISEP's AFFINIMIP® technology provides cleaner extracts thanks to high binding affinities for specific target molecule(s), resulting in lower limits of detection and quantification. The selectivity of AFFINIMIP® SPE allows stringent washing steps in order to remove all interferents and therefore minimize matrix effects. AFFINIMIP® SPE products enable rapid, highly specific and sensitive detection of the target analyte from complex matrices.

Ready for use analytical method

All AFFINIMIP® SPE products are delivered with an instruction sheet, describing the sample preparation and clean-up method in detail, recommended analytical conditions and a quality assurance certificate. Developed by AFFINISEP's Analytical Department, the extraction protocol will in most cases require no further optimization. No extra equipment than the usual required for conventional SPE is necessary.

Stable at broad pH ranges and high temperatures

AFFINIMIP® SPE phases are highly cross-linked polymers that maintain stability when exposed to organic solvents. They are stable at high temperatures, and can be used over broad pH ranges, without loss of selectivity. They can be stored at room temperatures for prolonged periods of times (several years). This is extremely advantageous over immunoaffinity based products.

Reduction of ion suppression

Ion suppression is a problematic phenomenon that occurs when analyzing trace levels of analyte(s) in complex matrices with hyphenated techniques such as LC-MS-MS (Liquid Chromatography - tandem Mass Spectrometry). It is caused by the co-elution of matrix components with analyte(s) of interest and negatively affects several analytical figures of merit such as detection capability, precision and accuracy. Therefore, a perfect sample cleanup during sample preparation is critical. Thanks to the selectivity of AFFINIMIP® technology, ion suppression is reduced compared to more conventional SPE phases.