Specific extraction of Fumonisins and zearalenone from maize products

Zearalenone (ZON) and Fumonisins are mycotoxins produced by Fusarium molds. Zearalenone is known to cause estrogenic effects at relatively low levels, including infertility, reduced serum testosterone levels and sperm counts, reduced incidence of pregnancy, and change in progesterone levels. In addition, Zearalenone can delay the breeding process and cost the producer significant economic and physical losses.

Fumonsins are known to cause animal diseases and is suspected human carcinogens. Fumonisin B1 is the most abundant member of the family of Fumonisins while Fumonisin B2 is more cytotoxic than fumonisin B1.

Several countries have instituted Zearalenone and Fumonisins B1+B2 restrictions in foodstuffs. In Europe, Regulation (EC) 1126/2007 sets maximum levels for Zearalenone at 350µg/kg in unprocessed maize, 100µg/kg in maize for human consumption and 20µg/kg in maize based food for infants and young children. Regarding Fumonisins (B1+B2) maximum allowable levels are 4000µg/kg in unprocessed maize, 1000µg/kg in maize for human consumption, and 200µg/kg in maize based food for infants and young children.

AFFINIMIP® SPE FumoZON are selective solid phase extraction cartridges that clean and concentrate Zearalenone and Fumonisins B1+B2 prior to analysis by HPLC-MS.


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  • Ready for use and optimized extraction methodology adapted to maize matrices
  • Perfect clean-up system for trace analysis of Fumonisins B1+B2 and Zearalenone
  • Very simple protocol
  • Compliant with European Commission Regulation (EC) 1126/2007

Main available formats

Designation, Format of cartridges, amount of sorbent

Box composition



Open cartridge 3mL 100mg 

25 Cartridges


50 Cartridges


 Fumonisin B1 standard solution - 1mL - 50µg/mL

1mL vial


Fumonisin B2 standard solution - 1mL - 50µg/mL

1mL vial


Mix Fumonisin B1/B2 standard solution - 1mL - 50µg/mL each

1mL vial


Zearalenone standard solution - 1mL - 100µg/mL

1mL vial


Specific formats for automates

AFFINIMIP®SPE FumoZON can be used with automates with the appropriate reference.

Designation, Format of cartridges Box composition Reference
AFFINIMIP® SPE FumoZON for Multipurpose sampler automate (GERSTEL)-3mL

50 cartridges



50 cartridges


Other formats can be supplied on demand

Application notes

Maize and maize-based baby food

Simultaneous analysis of Zearalenone and Fumonisins has been carried out on maize flour and maize based baby food after cleanup with AFFINIMIP® SPE FumoZON. Strong ion-suppression reduction and recoveries higher than 80% compliant with the performance criteria established by the European Commission Regulation (EC 401/2006) were obtained.

Application note AFFINIMIP® SPE FumoZON in Maize and Maize-based Baby Food


AFFINIMIP® SPE Cartridge VS ImmunoAffinity Column

Discover the advantages of AFFINISEP's Molecularly Imprinted Polymers over Immuno-affinity sorbents for mycotoxins analysis.


Fumonisins and their masked forms in maize products, M. Bryła, M. Roszko, K. Szymczyk, R. Jędrzejczak, E. Słowik,  W. Mieczysław, M. W. Obiedziński, Food Control, 59, 619 - 627, 2016. NEW!

Effect of Baking on Reduction of Free and Hidden Fumonisins in Gluten-free Bread, M. Bryła, M. Roszko, K. Szymczyk, R. Jędrzejczak, E. Słowik, M. W. Obiedziński, J. Agric. Food Chem, 62 (42), 10341-10347, 2014, DOI:10.1021/jf504077m

Application of molecularly imprinted polymers to determine B1, B2, and B3 fumonisins in cereal products, M. Bryła, R. Jędrzejczak, M. Roszko, K. Szymczyk, M. W. Obiedziński, J. Sękul, M. Rzepkowska, J. Sep. Sci., 36 (3), 578-584, 2013.


Multimycotoxin and single mycotoxin detections using a new SPE sorbent based on Molecularly Imprinted Polymers, D. Derrien, M. Mulet, B. Chevalier, F. Alix, C. Pérollier, O. Lépine, S. Bayoudh, K. Naraghi, 125th AOAC, New orleans, Louisiane (USA), 18 - 21 Sept. 2011.


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