AFFINIMIP® SPE Patulin

Extraction of Patulin from a variety of apple-based food matrices : Apple juice, puree, cider, baby food.

Patulin is a mycotoxin produced by a variety of molds, particularly Aspergillus and Penicillium. It is commonly found in rotting apples, and the amount of patulin in apple products is generally viewed as a measure of the quality of the apples used in production.

Studies have shown that it is genotoxic. Several countries have instituted patulin restrictions in apple products. The World Health Organization, the FDA and the European Union recommend a maximum concentration of 50µg/L in apple juice and 25µk/kg in apple puree. Member countries of the European Union have set maximum allowable levels of patulin at 10μg/kg in apple juice and solid apple products, including apple compote and apple puree, for infants and young children (European Commission Regulation (EC) 1881/2006).

AFFINIMIP® SPE Patulin are selective solid phase extraction cartridges that selectively clean and concentrate the toxin prior to analysis by HPLC.

 

We sell different kits such as :

And also a strong support for the implementation of the method, numerous Application Notes, video etc ...

 

        

 

 

Benefits

  • Ready for use and optimized extraction protocol
  • Perfect clean-up system for trace analysis of Patulin
  • Enrichment of the analyte
  • Applicable to several apple derived matrices
  • Removal of main matrix interferent: 5-Hydroxymethylfurfural (HMF)
  • Compliant with European Commission Regulation (EC) 1881/2006
 

Booklet AFFINIMIP® SPE Patulin

Our Booklet AFFINIMIP®SPE Patulin collects main useful information about :

  • Regulations concerning Patulin
  • AFFINIMIP®SPE Patulin description
  • Proficiency testing obtained with this product
  • Product added-value vs alternative methods
  • Application notes
  • Relative products (standard solution...)

 

Main available formats

Designation, Format of cartridges, amount of sorbent

Box composition

Reference

AFFINIMIP®SPE Patulin  for apple juice or apple puree

Open cartridge 3mL 100mg 

25 Cartridges

FS102-02

50 Cartridges

FS102-03

AFFINIMIP®SPE Patulin  for dried apple, apple juice, apple puree, concentrate and thick fruit juice 

Open cartridge 6mL 200mg 

25 Cartridges

FS102-02B-200mg

50 Cartridges

FS102-03B-200mg

Pectinase enzymatic solution 

50mL Pectinase

REA-001-50mL

Kit Pectinase & AFFINIMIP®SPE Patulin  for apple juice or apple puree

50mL Pectinase solution + open cartridges 3mL 100mg

25 Cartridges + 50 mL Pectinase

FS102-02K

50 Cartridges + 50 mL Pectinase

FS102-03K

Kit Pectinase & AFFINIMIP®SPE Patulin   for dried apple, apple juice, apple puree, concentrate and thick fruit juice

50mL Pectinase solution + open cartridges 6mL 200mg

25 Cartridges + 50 mL Pectinase

FS102-02KB-200mg

50 Cartridges + 50 mL Pectinase

FS102-03KB-200mg

Patulin standard solution - 1mL -100µg/mL

1mL vial

REA-PAT-1mL

HPLC column

150x 2.1mm

LC-pat-150.2.1

AFFINIMIP® SPE Patulin & HPLC column

50 cartridges of 3mL + 1 HPLC column

 

50 cartridges of 6mL + 1 HPLC column

FS102-03KLC

 

FS102-03BKLC-200mg

AFFINIMIP® SPE Patulin, HPLC column & standard

50 cartridges 3mL+ 1 HPLC column& Standard

FS102-03KLCS

AFFINIMIP® SPE Patulin, HPLC column, pectinase & standard

50 cartridges 3mL + 1 HPLC column, pectinase & standard

FS102-03BKLCSP-200mg

 

 

Specific formats for automates

AFFINIMIP®SPE Patulin can be used with automates with the appropriate reference.

Designation, Format of cartridges Box composition Reference
AFFINIMIP®SPE Patulin for Multipurpose sampler automate (GERSTEL)-3mL

50 cartridges

FS102-03-GERSTEL

AFFINIMIP®SPE Patulin for Multipurpose sampler automate (GERSTEL) -6mL

50 cartridges

FS102-03B-GERSTEL

AFFINIMIP®SPE Patulin for ASPEC automate (GILSON)-3mL

50 cartridges

FS102-03
AFFINIMIP®SPE Patulin for ASPEC automate (GILSON)-6mL

50 cartridges

FS102-03B-200mg
 

Boxes of 25 or 50 cartridges are supplied with a certificate of analysis and instructions for use describing the protocol to carry out the analysis.

Other formats can be supplied on demand

 

Application notes

All application notes are gathered in the Booklet AFFINIMIP®SPE Patulin

LC-MS/MS detection New!

Combination of AFFINIMIP® SPE PATULIN clean-up with LC-MS/MS detection strongly shorten Patulin analysis with excellent recoveries and repeatability!

Pear & apple puree as well as apple juice protocols were developed.

Learn more : 

Application note AFFINIMIP® SPE PATULIN - Pear & Apple Puree - LC-MS/MS

Application note AFFINIMIP® SPE PATULIN - Apple Juice - LC-MS/MS

 

Apple juice & apple puree -  UV detection

Apple juice and apple puree spiked with Patulin were tested on a HPLC system after sample cleanup with AFFINIMIP®SPE Patulin. The chromatograms obtained show that the main matrix interferent, 5-Hydroxymethylfurfural (HMF) is removed. Average Recoveries of 90.6% were obtained in apple juice spiked at 40μg/kg and 84% in apple puree spiked at 20μg/kg. Recoveries > 80% were also obtained in baby food at maximum allowable level for infants and young children (10μg/kg).

Learn more : Application note AFFINIMIP® SPE PATULIN Apple Juice and Apple Puree
                     Application note AFFINIMIP® SPE PATULIN Baby Food

 

Apple-fruit puree -  UV detection

Apple/pear and apple/strawberry purees were tested on a HPLC system after sample cleanup with AFFINIMIP®SPE Patulin. Recoveries above 75% were obtained.

Application note AFFINIMIP® SPE Patulin apple-fruit puree

Cider -  UV detection

Cider spiked with Patulin at 10µg/kg and 40 µg/kg was tested on a HPLC system after sample cleanup with AFFINIMIP® SPE Patulin. Clean chromatograms and recoveries above 80% were obtained.

Application note AFFINIMIP®SPE Patulin in cider

Automated protocol -  UV detection

The combination of AFFINIMIP®SPE Patulin cartridges with an automate GILSON GX-271 ASPEC™ shows accurate and reliable analysis with recovery yields higher than 80% on a apple juice. 

Application note AFFINIMIP®SPE Patulin automated protocol

Dried apple with AFFINIMIP®SPE Patulin 6mL-200mg -  UV detection

dried apple dices spiked with Patulin at 10µg/kg and 20 µg/kg were tested on a HPLC system after sample cleanup with AFFINIMIP®SPE Patulin 200mg cartridges. Clean extracts and recoveries above 90% were obtained.

Application note AFFINIMIP®SPE Patulin in dried apple

Thick juices and fruit juice concentrates with AFFINIMIP®SPE Patulin 6mL-200mg -  UV detection

Patulin could be analyzed on thick juices and fruit juice concentrates with recovery yields higher than 80% after a pectinase treatment and an efficient and fast clean-up with AFFINIMIP®SPE Patulin.

Application note AFFINIMIP® SPE Patulin in Thick juices and fruit juice concentrate

Posters and Publications

- In this article, National R&D institute for Food Bioresources of Bucharest has studied the thermal degradation of Patulin during the process to obtain apple puree and nectar. The determination of patulin was carried out by a clean-up with AFFINIMIP SPE Patulin - 6mL based on sample preparation describes on AFFINISEP application notebook or Patulin booklet for respectively whole apple and Apple puree/Nectar. Repeatability (<10%), recovery yields and limit of detection (<1.4µg/kg) and quantification (<4.6µg/kg) were determined for each matrice.

Assessment of the Thermal Stability of Patulin in Apple Puree and of Possibilities for Reduction of Patulin Contamination Level Through Apple Processing, M. Catana, L. Catana, E. Iorga, A. Culetu, V. Ionescu, N. Belc, Revista de Chimie, 69 (4), 809 - 814, 2018 (FREE ACCESS)

- Italian researchers from the University of Udine have developed a method for the analysis of patulin from apple juice, puree and jam samples with an extraction with AFFINIMIP®SPE PATULIN prior to UPLC-UV analysis. With high recoveries (>77%), the method was validated and found to be linear in the range 2-100 μg kg−1 with correlation coefficients greater than 0.965 and repeatability relative standard deviation below 11% in all cases. Compared with QuEChERS method and C18, AFFINIMIP®SPE PATULIN showed higher recoveries and selectivity for patulin.

Selective solid-phase extraction using a molecularly imprinted polymer for the analysis of patulin in apple-based foods, Lucci, P., Moret, S., Bettin, S. and Conte, L., J. Sep. Science., 40 (2), 458-465, 2017. doi:10.1002/jssc.201601009

- An analytical method was developed and validated for determination of patulin in apple puree by HPLC with a clean-up with AFFINIMIP®SPE PATULIN cartridges. The recovery in the range of 5 μg/kg-80 μg/kg was 81.47%. The limit of detection (LOD) was 1.36 μg/kg, and the limit of quantification (LOQ) was 4.55 μg/kg.

Assessment of Patulin Content in Apple Puree and Apple and Fruit Puree by High Performance Liquid Chromatography, M. Catana, L. Catana, E. Iorga, M. Negoita, V. Ionescu, N. Belc, D. Efstatiade, H. Y. Aboul-Enein, J. Envir. Sci. Eng. A 5, 371-381, 2016. doi:10.17265/2162-5298/2016.07.005 (FREE ACCESS)

- Analysis of Patulin in apple juice using the Chromaster HPLC System with PDA detector, Application note C-12125 of March 2014 developed by VWR and published in the Chromjournal of March 2015.

- Automated analysis of Patulin in Apple juice

Automatisierte Anwendung von Affinimip -SPE-Säulen bein der Bestimmung von Patulin in Apfelsaft, Maria Barricelli, Deutsche Lebensmittel-Rundschau : DLR ; Analytik, Forschung, Prozesse, Recht Vol. 110, No. 7 (2014), p. 310-315 (In german)

The translation of the title is 'Automated application of Affinimip -SPE columns for the determination of patulin in apple juice'.

At the Berlin Brandebourg State Laboratory (LLBB), Mrs Maria Barricelli implemented and validated an automated method for Patulin analysis in apple juice using AFFINIMIP®SPE Patulin in combination with an ASPEC automate to replace their time consuming Liquid Liquid Extraction process. The method was confirmed with certified materials (Apple Juice FAPAS T1650QC, DLA 17/2012) and is now routinely used in the laboratory.

- Modelling of patulin photo-degradation by a UV multi-wavelength emitting lamp, R. Ibarz, A. Garvín, V. Falguera, J. Pagán, S. Garza, A. Ibarz, Food Research International 66 (2014) 158-166

- Breakthrough innovation in rapid detection kits using MOLECULARLY IMPRINTED POLYMERS SPE for an early quantification of PATULIN from apple-based food matrices, D. Derrien, C. Pérollier, O. Lépine, K. Naraghi, S. Bayoudh, poster at 7th World Mycotoxin Forum, Rotterdam, The Netherlands, November 5-9, 2012.

- Molecularly Imprinted Polymer for Solid Phase Extraction of Patulin mycotoxin, D. Derrien, M. Mulet, F. Alix, C. Pérollier, O. Lépine, K. Naraghi, S. Bayoudh, 33rd Mycotoxin Workshop, Freising, Germany, 30 May - 1 June 2011

For other AFFINIMIP® SPE Cartridges please click here

 

Pectinase

REA-001-50ML Pectinase 50mL
Reference : REA-001-50mL
For more information


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