AFFINIMIP® SPE Zearalenone

Specific extraction of Zearalenone from a variety of food samples matrices: cereals, baby food.

Zearalenone is a mycotoxin produced as a secondary metabolite of various Fusarium fungi. Zearalenone is known to cause estrogenic effects at relatively low levels, including infertility, reduced serum testosterone levels and sperm counts, reduced incidence of pregnancy, and change in progesterone levels. In addition, Zearalenone can delay the breeding process and cost the producer significant economic and physical losses.

In Europe, Regulation (EC) 1881/2006 sets maximum levels for Zearalenone in foodstuffs such as processed cereal-based food and baby foods at 20µg/Kg.

AFFINIMIP® SPE Zearalenone are selective solid phase extraction cartridges that selectively clean and concentrate the toxin prior to analysis by HPLC.


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  • Ready for use and optimized extraction methodologies adapted to several matrices
  • Perfect clean-up system for trace analysis of Zearalenone
  • Very simple protocol
  • Compliant with European Commission Regulation (EC) 1881/2006

Main available formats

Designation, Format of cartridges, amount of sorbent

Box composition


AFFINIMIP® SPE Zearalenone   Open cartridge 3mL 100mg 

50 Cartridges


Zearalenone standard solution - 1mL - 100µg/mL

1mL vial


Specific formats for automates

AFFINIMIP®SPE Zearalenone can be used with automates with the appropriate reference.

Designation, Format of cartridges Box composition Reference
AFFINIMIP®SPE Zearalenone for Multipurpose sampler automate (GERSTEL)-3mL

50 cartridges


AFFINIMIP®SPE Zearalenone for ASPEC automate (GILSON)-3mL

50 cartridges


Other formats can be supplied on demand

Application notes

AFFINIMIP® SPE Cartridge VS ImmunoAffinity Column

Discover the advantages of AFFINISEP's Molecularly Imprinted Polymers over Immuno-affinity sorbents for mycotoxins analysis.

Analysis of Zearalenone in Meat New!

The extraction of Zearalenone in Meat was carried out with AFFINIMIP®SPE Zearalenone before analysis. 

Application note AFFINIMIP® SPE Zearalenone - Meat

Cereal products and baby food

Zearalenone analysis has been carried out on maize (41µg/Kg), rice (41µg/Kg) and cereal-based baby food (10 and 41µg/Kg). Very simple and clean chromatograms were obtained after a clean up with AFFINIMIP® SPE Zearalenone.

Application note AFFINIMIP® SPE Zearalenone Cereal Products and Baby Food

Edible corn oil

This method uses a very quick sample preparation by simply diluting corn oil in a solvent before cleanup. Cleanup with AFFINIMIP®SPE Zearalenone provides clear and unambiguous results at a broad range of concentration levels. The tests performed demonstrate high recovery yields with a low background.

Application note: Selective Solid Phase Extraction of Zearalenone from Edible Corn Oil using AFFINIMIP® SPE

Automated protocol

The combination of AFFINIMIP® SPE Zearalenone cartridges with an automate GILSON GX-271 ASPEC™ shows accurate and reliable analysis with recovery yields higher than 80% on a wheat sample. 

Application note AFFINIMIP® SPE Zearalenone Automated Protocol (GILSON GX271 ASPEC™)


Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts, P. Lucci, D. Derrien, F. Alix, C. Pérollier, S. Bayoudh, Analytica Chimica Acta, 672, 15-19, 2010.


Selective Solid Phase Extraction of Zearalenone from Edible Corn Oil using Molecularly Imprinted Polymers, D. Derrien, B. Chevalier, F. Alix, C. Pérollier, Ol. Lépine, S. Bayoudh, K. Naraghi, 7th World Mycotoxin Forum, Rotterdam, The Netherlands, November 5-9, 2012



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