On-line SPE

On-line SPE columns for coupling with LC analysis

SPE technique can be coupled on-line to HPLC for a high sensitivity or for limited amount of sample. 

ON-LINE SPE PROCEDURE STEPS

An on-line SPE column containing the SPE sorbents is coupled within the injection loop of a six-port rotary valve. The on-line SPE column is reusable.

A three steps process is used:

1- Sample injection: The valve is configured with the injector directly in contact with the on-line SPE column. The sample is injected and goes through the on-line  SPE column where the analytes remain.

2- Washing: a solution is used to wash out most interferences.

3- Analysis:  The valve is switched. The analytes are eluted out of the sorbent by the LC mobile phase and transfered into the analytical column for their analyses.

 

 On-line SPE process

 

 

On-line SPE columns for hydrophilic - hydrophobic polymers

 

Product

 

Product reference

 

Nber of columns

 

I.D. (mm)

Lenght (mm)

 

On-line AttractSPETM W/O

SPE columns

 

 

OnlineSPE-HLB-1.5.20

1

4.6

20

OnlineSPE-HLB-1.2.20

1

2.1

20

 

For other on-line SPE products, please contact us

AFFINISEP can provide you with on-line SPE of all products on demand

 

 


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