AFFINIMIP® SPE 18F - Aromatic Nucleophilic Substitution

Purification of 18F radiotracer obtained from ammonium precursor by Aromatic Nucleophilic Substitution.

AFFINIMIP® SPE for purification of radiotracers is based on the affinity for the precursor. In the case of Aromatic Nucleophilic Substitution with 18F, impurities, Kryptofix and residual 18F are also retained on the cartridge, while the purified radiotracer is obtained after elution.








AFFINIMIP® SPE 18F - Aromatic Nucleophilic Substitution is a selective solid phase extraction cartridge used to purify rapidly and perfectly a 18F radiotracer synthesized from an aromatic nucleophilic substitution. The cartridge retains the ammonium-based precursor as well as the phenol- and dialkylamino-based impurities.



- Validated for radiotracers (FBA, Ethyl 4-Fluorobenzoate) issued from a SNAr with an ammonium precursor

- Rapid and simple method purification: No need for a preparative HPLC thanks to our All-in-One SPE

- Improved radiotracer purity: No traces of the 3 unwanted products (precursor and by-products) and low traces of Kryptofix and residual 18F were detected.

- Increased reliability of radiotracer production: Reduction of manual handling steps

- No cross contamination thanks to single use cartridges

Application notes

Fluoration of 4-Trimethylammoniumbenzaldehyde Tosylate and Purification of 4-Fluorobenzaldehyde (FBA) using AFFINIMIP® SPE 18F - Aromatic Nucleophilic Substitution

Fluoration of Ethyl 4-Trimethylammoniumbenzoate Iodide and Purification of Ethyl 4-Fluorobenzoate using AFFINIMIP® SPE 18F - Aromatic Nucleophilic Substitution

Posters and articles

- Development & automation of a nove l[18F]F prosthetic group, 2-[18F]-fluoro-3-pyridinecarboxaldehyde, and its application to an amino(oxy)-functionalised Aβ peptide, O. Morris, J. Gregory, M. Kadirvel, F. Henderson, A. Blykers, A. Mc Mahon, M. Taylor, D. Allsop, S. Allan, J. Grigg, H. Boutin, C. Prenant, Applied RadiationandIsotopes, 116 (2016) 120-127. (Open access article) NEW!









In this article, the authors describe the automated synthesis of [18F]FPCA peptide, a novel, water-soluble prosthetic group using AFFINIMIP®SPE 18F - Aromatic Nucleophilic Substitution purification. It's radiochemistry has been developed and fully-automated for application in chemoselective radiolabelling of amino(oxy)-derivatised RI-OR2-TAT peptide, (Aoa-k)-RI-OR2-TAT, using a GE TRACERlab FX-FN. RI-OR2-TAT is a brain-penetrant, retro-inverso peptide that binds to amyloid species associated with Alzheimer's Disease.

- PET Imaging of Macrophage Mannose Receptor-Expressing Macrophages in Tumor Stroma Using 18F-Radiolabeled Camelid Single-Domain Antibody Fragments, A. Blykers, S. Schoonooghe, C. Xavier, K. D'hoe, D. Laoui, M. D'Huyvetter, I. Vaneycken, F. Cleeren, G. Bormans, J. Heemskerk, G. Raes, P. De Baetselier, T. Lahoutte, N. Devoogdt, J. A. Van Ginderachter, and V. Caveliers, J Nucl Med, 2015; 56:1265-1271 DOI: 10.2967/jnumed.115.156828 (Open access article) NEW!

The prosthetic group N-succinimidyl-4-18F-fluorobenzoate (18F-SFB) was purified with AFFINIMIP®SPE 18F - Aromatic Nucleophilic Substitution.


- Preclinical validations of [18F]FPyPEGCBTc(RGDfK): a 18F-labelled RGD peptide prepared by ligation of 2-cyanobenzothiazole and 1,2-aminothiol to image angiogenesis, D. J. Colin, J. A. H. Inkster, S. Germain and Y. Seimbille,EJNMMI Radiopharmacy and Chemistry, 2016, 1:16 DOI: 10.1186/s41181-016-0019-z (Open access article) NEW!


- A novel 2-cyanobenzothiazole-based 18F prosthetic group for conjugation to 1,2-aminothiol-bearing targeting vectors J. A. H. Inkster, D. J. Colin and Y. Seimbille, Org. Biomol. Chem., 2015,13, 3667-3676 DOI: 10.1039/C4OB02637C

preparative synthesis of [18F]FPyPEGCBT





In this article, the authors describe the preparative synthesis of [18F]FPyPEGCBT for which its HPLC purification was replaced by AFFINIMIP®SPE 18F - Aromatic Nucleophilic Substitution purification with a DMF elution of the product of interest. The authors conclude that the advantage of such purification are "the obviation of a time-consuming HPLC step and a significant simplication of the overall radiopharmaceutical protocol."

- 18F-Anti-MMR-nanobodies for PET imaging of tumor-associated macrophage subtypes as surrogate markers for tumor hypoxia, Anneleen Blykers, Catarina Xavier, Ilse Vaneycken, Damya Laoui, Nick Devoogdt, Matthias D'huyvetter, Tony Lahoutte, Jo A. Van Ginderachter and Vicky Caveliers, European Molecular Imaging Meeting (EMIM), Turin, Italy, 26-28 May 2013. In this work, N-succinimidyl-4-18F-fluorobenzoate (18F-SFB) was successfully purified after synthesis with AFFINIMIP®SPE 18F - Aromatic Nucleophilic Substitution.

- Rapid Purification of Fluorine-18 Containing Synthons Using Molecularly Imprinted Polymer Cartridges, G. Smith, S. Bayoudh, C. Pérollier, R. Bhalla, International Symposium on Radiopharmaceutical Sciences (ISRS), Jeju, Korea, 12-17 May 2013. Works carried out in collaboration with GE Healthcare.

- Fast and Selective Purification of a Fluorous Radiotracer using SPE based on Molecularly Imprinted Polymers, C. Pérollier, F. Alix, S. Bayoudh, K. Naraghi, European Association of Nuclear Medicine (EANM), Milan, Italy, October 27-31, 2012.






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