Peptide desalting

Purification and desalting of peptides in bottom-up proteomic approaches.

BioSPE^® PurePep is used for efficient peptide desalting after enzymatic digestion, prior to LC-MS/MS analysis. Up to 97% proteins are identified after desalting of HeLa digest on BioSPE^®PurePep, with a good recovery of peptides on the whole polarity range, and good repeatability (RSD < 3%).

For desalting of the most hydrophilic peptides, we recommend the use of BioSPE^® PurePep Broad, that can efficiently retain small polar peptides. BioSPE^® PurePep Broad is also stable under extreme pH conditions (pH 0-14) and can run dry during the SPE process without consequences on your results.

The BioSPE® PurePep and BioSPE® PurePep Broad kits are composed of:

Desalting columns
Adapters (for tips centrifugation) and/or collection tools (centrifuge tubes or collection plates). Each kit contains two collection tools (one for the conditioning/loading/washing steps and one for the elution step)
A detailed protocol for fast and easy desalting

To determine which format and capacity will best suit your needs, please refer to the tables in the “Formats & Capacities” tab.

Products list

Product Name	Product Description	Product Price	Cart Actions
BioSPE™ PurePep – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep.S.1	96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep.H.1	96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep-Broad.H.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate & collection plates Ref. : KT-Coll-96W-PurePep-Broad.S.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96	Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96	Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep.H.1	96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep.S.1	96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep-Broad.H.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates Ref. : KT-Coll-µ96W-PurePep-Broad.S.1	96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.S.10.96	Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 10µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.H.10.96	Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters Ref. : KT-Adapt-Tips-PurePep.SC.10.96	Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep.S.1000.96	Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 1mL & spin adapters Ref. : KT-Adapt-Tips-PurePep.H.1000.96	Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.S.200.96	Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk \| Spin adapters - 96/pk \| 2mL collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Tips 200µL & spin adapters Ref. : KT-Adapt-Tips-PurePep.H.200.96	Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 3mL Ref. : CAR3-PurePep-Broad.H.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin Ref. : Spin-PurePep.S.50	Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 3mL Ref. : CAR3-PurePep-Broad.S.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 6mL Ref. : CAR6-PurePep-Broad.H.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 6mL Ref. : CAR6-PurePep-Broad.S.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin Ref. : Spin-PurePep-Broad.S.50	Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin Ref. : Spin-PurePep-Broad.H.50	Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 15mL Ref. : Spin15-PurePep-Broad.S.25	Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 15mL Ref. : Spin15-PurePep-Broad.H.25	Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 50mL Ref. : Spin50-PurePep-Broad.H.25	Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Spin 50mL Ref. : Spin50-PurePep-Broad.S.25	Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – µSpin Ref. : µSpin-PurePep-Broad.S.50	µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – µSpin Ref. : µSpin-PurePep-Broad.H.50	µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – µSpin Ref. : µSpin-PurePep.S.50	µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 1mL Ref. : CAR1-PurePep.S.50	SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 1mL Ref. : CAR1-PurePep.H.50	SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 3mL Ref. : CAR3-PurePep.S.50	SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 3mL Ref. : CAR3-PurePep.H.50	SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 6mL Ref. : CAR6-PurePep.S.50	SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Cartridges 6mL Ref. : CAR6-PurePep.H.50	SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin Ref. : Spin-PurePep.H.50	Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 15mL Ref. : Spin15-PurePep.H.25	Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 15mL Ref. : Spin15-PurePep.S.25	Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 50mL Ref. : Spin50-PurePep.H.25	Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – Spin 50mL Ref. : Spin50-PurePep.S.25	Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep – µSpin Ref. : µSpin-PurePep.H.50	µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 1mL Ref. : CAR1-PurePep-Broad.H.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk	SignIn To View	SignIn To View
BioSPE™ PurePep Broad – Cartridges 1mL Ref. : CAR1-PurePep-Broad.S.50	SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk	SignIn To View	SignIn To View