Peptide desalting
Purification and desalting of peptides in bottom-up proteomic approaches.
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BioSPE® PurePep is used for efficient peptide desalting after enzymatic digestion, prior to LC-MS/MS analysis. Up to 97% proteins are identified after desalting of HeLa digest on BioSPE® PurePep, with a good recovery of peptides on the whole polarity range, and good repeatability (RSD < 3%).
For desalting of the most hydrophilic peptides, we recommend the use of BioSPE® PurePep Broad, that can efficiently retain small polar peptides. BioSPE® PurePep Broad is also stable under extreme pH conditions (pH 0-14) and can run dry during the SPE process without consequences on your results.
The BioSPE® PurePep and BioSPE® PurePep Broad kits are composed of:
- Desalting columns
- Adapters (for tips centrifugation) and/or collection tools (centrifuge tubes or collection plates). Each kit contains two collection tools (one for the conditioning/loading/washing steps and one for the elution step)
- A detailed protocol for fast and easy desalting
To determine which format and capacity will best suit your needs, please refer to the tables in the “Formats & Capacities” tab.
- No storage constraint (store dry at room temperature)
- Different formats available to adapt to sample volume and throughput (StageTips, Spin columns, 96 wellplates and SPE cartridges)
- Two capacities available for each format depending on your peptide amount
Details regarding the loading and elution volumes for our different BioSPE™PurePep products to find the best product depending on your sample volume.
Information table regarding the capacities (maximal amount of peptides/proteins that can be retained) of our different BioSPE™PurePep products :
- For Standard capacity, product references contain the letter S (e.g. Tips-PurePep.S.200.96),
- For High capacity, product references contain the letter H (e.g. Tips-PurePep.H.200.96).
Literature
Hijazi, H. et al. (2025) ‘Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms’, Journal of Proteome Research.
In their latest publication, Hassan M. Hijazi et al. investigate the identification of mouse-specific histone variants H3mm7 and H3mm13 from brain and testis extracts using LC-MS/MS.
To enable accurate detection of post-translational modifications such as acetylation and lactylation, peptide purification was performed with Affinisep BioSPE® PurePep, which proved highly effective in removing interfering substances and preserving modified peptides.
This approach improved MS/MS spectral quality, particularly for critical ions like b1 and CycIm, and helped address challenges related to isobaric variants caused by uncontrolled modifications such as propionylation.
Product used : BioSPE® PurePep Tips 200µl (Catalog # KT-Adapt-Tips-PurePep.H.200.96)
Application notes
- Proteomics
Estimation of the working range on BioSPE™PurePep SPE Tips – Standard capacity
New !
Peptide desalting with BioSPE™ PurePep
Products list
Product Image | Product Name | Product Description | Product Price | Cart Actions | |
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BioSPE™ PurePep – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep.S.1 |
96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep.H.1 |
96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep Broad – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep-Broad.H.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep Broad – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep-Broad.S.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep Broad – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep.H.1 |
96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep.S.1 |
96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep-Broad.H.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep-Broad.S.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.10.96 |
Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.10.96 |
Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters
Ref. : KT-Adapt-Tips-PurePep.SC.10.96 |
Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.1000.96 |
Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.1000.96 |
Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.200.96 |
Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk | Spin adapters - 96/pk | 2mL collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.200.96 |
Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Cartridges 3mL
Ref. : CAR3-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk |
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BioSPE™ PurePep – Spin
Ref. : Spin-PurePep.S.50 |
Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Cartridges 3mL
Ref. : CAR3-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 6mL
Ref. : CAR6-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 6mL
Ref. : CAR6-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk |
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BioSPE™ PurePep Broad – Spin
Ref. : Spin-PurePep-Broad.S.50 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Spin
Ref. : Spin-PurePep-Broad.H.50 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Spin 15mL
Ref. : Spin15-PurePep-Broad.S.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 15mL
Ref. : Spin15-PurePep-Broad.H.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 50mL
Ref. : Spin50-PurePep-Broad.H.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 50mL
Ref. : Spin50-PurePep-Broad.S.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – µSpin
Ref. : µSpin-PurePep-Broad.S.50 |
µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – µSpin
Ref. : µSpin-PurePep-Broad.H.50 |
µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – µSpin
Ref. : µSpin-PurePep.S.50 |
µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – Cartridges 1mL
Ref. : CAR1-PurePep.S.50 |
SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 1mL
Ref. : CAR1-PurePep.H.50 |
SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 3mL
Ref. : CAR3-PurePep.S.50 |
SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 3mL
Ref. : CAR3-PurePep.H.50 |
SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 6mL
Ref. : CAR6-PurePep.S.50 |
SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 6mL
Ref. : CAR6-PurePep.H.50 |
SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk |
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BioSPE™ PurePep – Spin
Ref. : Spin-PurePep.H.50 |
Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – Spin 15mL
Ref. : Spin15-PurePep.H.25 |
Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 15mL
Ref. : Spin15-PurePep.S.25 |
Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 50mL
Ref. : Spin50-PurePep.H.25 |
Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 50mL
Ref. : Spin50-PurePep.S.25 |
Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – µSpin
Ref. : µSpin-PurePep.H.50 |
µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Cartridges 1mL
Ref. : CAR1-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 1mL
Ref. : CAR1-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk |
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