Peptide desalting

Purification and desalting of peptides in bottom-up proteomic approaches.

 

.

01 sf peptide desalting v2

BioSPE™ PurePep is used for efficient peptide desalting after enzymatic digestion, prior to LC-MS/MS analysis. Up to 97% proteins are identified after desalting of HeLa digest on BioSPE™PurePep, with a good recovery of peptides on the whole polarity range, and good repeatability (RSD < 3%).

For desalting of the most hydrophilic peptides, we recommend the use of BioSPE™ PurePep Broad, that can efficiently retain small polar peptides. BioSPE™ PurePep Broad is also stable under extreme pH conditions (pH 0-14) and can run dry during the SPE process without consequences on your results.

The BioSPE™ PurePep and BioSPE™ PurePep Broad kits are composed of:

  • Desalting columns
  • Adapters (for tips centrifugation) and/or collection tools (centrifuge tubes or collection plates). Each kit contains two collection tools (one for the conditioning/loading/washing steps and one for the elution step)
  • A detailed protocol for fast and easy desalting

To determine which format and capacity will best suit your needs, please refer to the tables in the “Formats & Capacities” tab.

Products list

Product Name Product Price Product SKU Cart Actions
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep – 96 wellplate & collection plates

96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep.S.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep – 96 wellplate & collection plates

96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep.H.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep Broad – 96 wellplate & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep-Broad.H.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep Broad – 96 wellplate & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep-Broad.S.1
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96
kit tips 1ml 600x600

BioSPE™ PurePep Broad – Tips 1mL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL -  Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96
kit tips 1ml 600x600

BioSPE™ PurePep Broad – Tips 1mL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96
kit tips 200ul 600x600

BioSPE™ PurePep Broad – Tips 200µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96
kit tips 200ul 600x600

BioSPE™ PurePep Broad – Tips 200µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep – 96 wellplate for microelution & collection plates

96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep.H.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep – 96 wellplate for microelution & collection plates

96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep.S.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep-Broad.H.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep-Broad.S.1
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL & spin adapters

Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.10.96
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL & spin adapters

Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.10.96
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.SC.10.96
kit tips 1ml 600x600

BioSPE™ PurePep – Tips 1mL & spin adapters

Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.1000.96
kit tips 1ml 600x600

BioSPE™ PurePep – Tips 1mL & spin adapters

Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.1000.96
kit tips 200ul 600x600

BioSPE™ PurePep – Tips 200µL & spin adapters

Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk | Spin adapters - 96/pk | 2mL collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.200.96
kit tips 200ul 600x600

BioSPE™ PurePep – Tips 200µL & spin adapters

Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.200.96
cartridge biospe 3ml 600x600

BioSPE™ PurePep Broad – Cartridges 3mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk

Ref. : CAR3-PurePep-Broad.H.50
kit spin mini 600x600

BioSPE™ PurePep – Spin

Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep.S.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep Broad – Cartridges 3mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk

Ref. : CAR3-PurePep-Broad.S.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep Broad – Cartridges 6mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk

Ref. : CAR6-PurePep-Broad.H.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep Broad – Cartridges 6mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk

Ref. : CAR6-PurePep-Broad.S.50
kit spin mini 600x600

BioSPE™ PurePep Broad – Spin

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep-Broad.S.50
kit spin mini 600x600

BioSPE™ PurePep Broad – Spin

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep-Broad.H.50
kit spin 15ml 600x600

BioSPE™ PurePep Broad – Spin 15mL

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep-Broad.S.25
kit spin 15ml 600x600

BioSPE™ PurePep Broad – Spin 15mL

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep-Broad.H.25
kit spin 50ml 600x600

BioSPE™ PurePep Broad – Spin 50mL

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep-Broad.H.25
kit spin 50ml 600x600

BioSPE™ PurePep Broad – Spin 50mL

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep-Broad.S.25
kit spin micro 600x600

BioSPE™ PurePep Broad – µSpin

µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep-Broad.S.50
kit spin micro 600x600

BioSPE™ PurePep Broad – µSpin

µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep-Broad.H.50
kit spin micro 600x600

BioSPE™ PurePep – µSpin

µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep.S.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep – Cartridges 1mL

SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk

Ref. : CAR1-PurePep.S.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep – Cartridges 1mL

SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk

Ref. : CAR1-PurePep.H.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep – Cartridges 3mL

SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk

Ref. : CAR3-PurePep.S.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep – Cartridges 3mL

SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk

Ref. : CAR3-PurePep.H.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep – Cartridges 6mL

SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk

Ref. : CAR6-PurePep.S.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep – Cartridges 6mL

SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk

Ref. : CAR6-PurePep.H.50
kit spin mini 600x600

BioSPE™ PurePep – Spin

Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep.H.50
kit spin 15ml 600x600

BioSPE™ PurePep – Spin 15mL

Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep.H.25
kit spin 15ml 600x600

BioSPE™ PurePep – Spin 15mL

Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep.S.25
kit spin 50ml 600x600

BioSPE™ PurePep – Spin 50mL

Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep.H.25
kit spin 50ml 600x600

BioSPE™ PurePep – Spin 50mL

Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep.S.25
kit spin micro 600x600

BioSPE™ PurePep – µSpin

µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep.H.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep Broad – Cartridges 1mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk

Ref. : CAR1-PurePep-Broad.H.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep Broad – Cartridges 1mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk

Ref. : CAR1-PurePep-Broad.S.50