On-line SPE
A fast and efficient way to cleanup samples on-line prior to analysis
Reusable columns
SPE technique can be coupled on-line to HPLC for a high sensitivity or for limited amount of sample.
An on-line SPE column containing the SPE sorbents is coupled within the injection loop of a six-port rotary valve. The on-line SPE column is reusable.
A three steps process is used:
1- Sample injection: The valve is configured with the injector directly in contact with the on-line SPE column. The sample is injected and goes through the on-line SPE column where the analytes remain.
2- Washing: a solution is used to wash out most interferences.
3- Analysis: The valve is switched. The analytes are eluted out of the sorbent by the LC mobile phase and transfered into the analytical column for their analyses.
- Columns available with 2.1mm or 4.6mm diameter
- Wide choice of particle sizes, and sorbent chemistries (AFFINIMIP®SPE Molecularly Imprinted Polymers, AttractSPE® polymeric or SilactSPE® silica-based sorbents).
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