Intact protein purification

BioSPE® for intact protein purification (PureProt) is used for the purification and desalting of intact proteins and large peptides (MW > 10kDa) from biological matrices (serum, plasma…) or food matrices (milk), prior to LC-MS/MS analysis.

The BioSPE® PureProt kit is composed of:

• Purification columns
• Adapters (for tips centrifugation) and/or collection tools (centrifuge tubes or collection plates). Each kit contains two collection tools (one for the conditioning/loading/washing steps and one for the elution step)
• A detailed protocol for easy and efficient clean-up of proteins

To determine which format and capacity will best suit your needs, please refer to the tables in the “Formats & Capacities” tab.

• High recovery yield
• No storage constraint (store dry at room temperature)
• Different formats available to adapt to sample volume and throughput (StageTips, Spin columns, 96 wellplates and SPE cartridges)
• Two capacities available for each format depending on your protein amount

Details regarding the loading and elution volumes for BioSPE® Intact protein purification (PureProt) to find the best product depending on your sample volume.

Information table regarding the capacities (maximal amount of peptides/proteins that can be retained) of our different BioSPE® PureProt products :

• For Standard capacity, product references contain the letter S (e.g. Tips-PureProt.S.200.96).
  For High capacity, product references contain the letter H (e.g. Tips-PureProt.H.200.96)