Intact protein purification
Purification of intact proteins in top-down proteomic approaches.
BioSPE™ PureProt is used for the purification and desalting of intact proteins and large peptides (MW > 10kDa) from biological matrices (serum, plasma…) or food matrices (milk), prior to LC-MS/MS analysis.
The BioSPE™ PureProt kit is composed of:
- Purification columns
- Adapters (for tips centrifugation) and/or collection tools (centrifuge tubes or collection plates). Each kit contains two collection tools (one for the conditioning/loading/washing steps and one for the elution step)
- A detailed protocol for easy and efficient clean-up of proteins
To determine which format and capacity will best suit your needs, please refer to the tables in the “Formats & Capacities” tab.
- High recovery yield
- No storage constraint (store dry at room temperature)
- Different formats available to adapt to sample volume and throughput (StageTips, Spin columns, 96 wellplates and SPE cartridges)
- Two capacities available for each format depending on your protein amount
Details regarding the loading and elution volumes for BioSPE™PureProt to find the best product depending on your sample volume.
Information table regarding the capacities (maximal amount of peptides/proteins that can be retained) of our different BioSPE™PureProt products :
- For Standard capacity, product references contain the letter S (e.g. Tips-PureProt.S.200.96).
For High capacity, product references contain the letter H (e.g. Tips-PureProt.H.200.96)
Products list
Product Name | Product Price | Product SKU | Cart Actions |
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BioSPE™ PureProt – 96 wellplate & collection plates96 Wellplate for protein purification - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk Ref. : KT-Coll-96W-PureProt.S.1 |
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9999 |
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BioSPE™ PureProt – 96 wellplate & collection plates96 Wellplate for protein purification - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk Ref. : KT-Coll-96W-PureProt.H.1 |
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9998 |
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BioSPE™ PureProt – 96 wellplate for microelution & collection plates96 Wellplate for protein purification - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk Ref. : KT-Coll-µ96W-PureProt.S.1 |
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9997 |
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BioSPE™ PureProt – 96 wellplate for microelution & collection plates96 Wellplate for protein purification - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk Ref. : KT-Coll-µ96W-PureProt.H.1 |
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9996 |
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BioSPE™ PureProt – Tips 10µL & spin adaptersKit of tips for protein purification - 10µL - Highd capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk Ref. : KT-Adapt-Tips-PureProt.H.10.96 |
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9995 |
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BioSPE™ PureProt – Tips 10µL & spin adaptersKit of tips for protein purification - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk Ref. : KT-Adapt-Tips-PureProt.S.10.96 |
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9994 |
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BioSPE™ PureProt – Tips 10µL Single Cell & spin adaptersKit of tips for protein purification in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk Ref. : KT-Adapt-Tips-PureProt.SC.10.96 |
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9993 |
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BioSPE™ PureProt – Tips 1mL & spin adaptersKit of tips for protein purification - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk Ref. : KT-Adapt-Tips-PureProt.S.1000.96 |
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9992 |
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BioSPE™ PureProt – Tips 1mL & spin adaptersKit of tips for protein purification - 1mL - Highd capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk Ref. : KT-Adapt-Tips-PureProt.H.1000.96 |
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9991 |
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BioSPE™ PureProt – Tips 200µL & spin adaptersKit of tips for protein purification - 200µL - Highd capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk Ref. : KT-Adapt-Tips-PureProt.H.200.96 |
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9990 |
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BioSPE™ PureProt – Tips 200µL & spin adaptersKit of tips for protein purification - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk Ref. : KT-Adapt-Tips-PureProt.S.200.96 |
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9989 |
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BioSPE™ PureProt – SpinSpin columns for protein purification - Standard capacity - 50/pk + collection tubes - 100/pk Ref. : Spin-PureProt.S.50 |
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9988 |
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BioSPE™ PureProt – SpinSpin columns for protein purification - High capacity - 50/pk + collection tubes - 100/pk Ref. : Spin-PureProt.H.50 |
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9987 |
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BioSPE™ PureProt – Cartridges 1mLSPE cartridges for protein purification - 1mL - Standard capacity - 50/pk Ref. : CAR1-PureProt.S.50 |
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9986 |
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BioSPE™ PureProt – Cartridges 3mLSPE cartridges for protein purification - 3mL - Standard capacity - 50/pk Ref. : CAR3-PureProt.S.50 |
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9985 |
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BioSPE™ PureProt – Cartridges 6mLSPE cartridges for protein purification - 6mL - Standard capacity - 50/pk Ref. : CAR6-PureProt.S.50 |
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9984 |
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BioSPE™ PureProt – Spin 15mLSpin columns for protein purification - 15mL - High capacity - 25/pk + collection tubes - 50/pk Ref. : Spin15-PureProt.H.25 |
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9983 |
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BioSPE™ PureProt – Spin 15mLSpin columns for protein purification - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk Ref. : Spin15-PureProt.S.25 |
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9982 |
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BioSPE™ PureProt – Spin 50mLSpin columns for protein purification - 50mL - High capacity - 25/pk + collection tubes - 50/pk Ref. : Spin50-PureProt.H.25 |
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9981 |
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BioSPE™ PureProt – Spin 50mLSpin columns for protein purification - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk Ref. : Spin50-PureProt.S.25 |
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9980 |
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BioSPE™ PureProt – µSpinµSpin columns for protein purification - Standard capacity - 50/pk + collection tubes - 100/pk Ref. : µSpin-PureProt.S.50 |
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9979 |
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BioSPE™ PureProt – µSpinµSpin columns for protein purification - High capacity - 50/pk + collection tubes - 100/pk Ref. : µSpin-PureProt.H.50 |
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9978 |
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BioSPE™ PureProt – Cartridges 1mLSPE cartridges for protein purification - 1mL - High capacity - 50/pk Ref. : CAR1-PureProt.H.50 |
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9977 |
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BioSPE™ PureProt – Cartridges 3mLSPE cartridges for protein purification - 3mL - High capacity - 50/pk Ref. : CAR3-PureProt.H.50 |
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9976 |
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BioSPE™ PureProt – Cartridges 6mLSPE cartridges for protein purification - 6mL - High capacity - 50/pk Ref. : CAR6-PureProt.H.50 |
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9975 |
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