Isolation of cell-free DNA and RNA

BioSPE™ PureGenNucleo cfDNA is a ready-to-use kit for the efficient isolation, purification, and concentration of circulating cell-free DNA and RNA from human plasma or urine samples.

Circulating cell-free DNA (ccfDNA or cfDNA) is a fragmented DNA that freely circulates in the bloodstream, without being associated with cells. Though it is not always the case, this DNA can be tumor-derived. In this case, cfDNA mostly originates from apoptotic cells.

If cfDNA usual concentrations in plasma range from 0.1ng DNA per mL to several hundred ng per mL of plasma, the total amount of circulating DNA and RNA in urine or plasma depends on several criteria, including but not limited to:

• Health condition of the donor
• Sampling method
• Handling of the sample
• DNA isolation method…

Composition of the kit

BioSPE™ PureGenNucleo cfDNA mini spin columns kit is sold in two packaging sizes (10/pk or 50/pk) containing the following elements:

We also provide the following two accessories in the kit: Reservoirs (200mL) and manifold adapters.

Other formats available

BioSPE™ PureGenNucleo cfDNA kit is also available with 96 well plates, ideal for high throughput and automated processing.

Other formats will be available soon:

• SPE Tips for single cell analysis: ideal for low input material and small sample volume < 1mL, with simple processing by centrifugation
• 15mL spin columns: ideal for large sample volumes up to 20mL

Don’t hesitate to contact us with any requests!

Benefits & features

• High recovery of fragmented DNA (≥ 50bp) and RNA
• Convenient processing of up to 5mL of sample at once thanks to the adapted reservoirs
• Small final elution volume (60µL) for highly concentrated DNA
• Purified DNA and RNA compatible with downstream PCR or cancer biomarker analysis
• Processing: vacuum (+ centrifugation for elution)
• Use: for research use only