Radiotracers purification
Find the best way to purify your radiotracers.
Purification is the critical step in radiotracer production.
Due to the short lifetime of radiotracers, the method of purification is a key aspect in radiopharmaceutical production and long purification steps must be avoided. The method of choice must also be effective enough to ensure a high level of radiochemical purity.
AFFINISEP proposes solutions based on Solid phase extraction (SPE).
SPE for Formulation :
- Switch from ACN/DMSO to Ethanol : AttractSPETM HLB
- Remove your polar by-products/impurities/salts : AttractSPETM HLB ; SilactTM SPE C18
SPE for 18F Separation :
- PS-DVB Carbonate : AttractSPETM SAX-HCO3
SPE for Purification :
- Purification of radiotracers obtained from Ammonium precursor : AFFINIMIP® SPE for purification of radiotracers is based on the affinity for the precursor. In the case of Aromatic Nucleophilic Substitution with 18F, impurities, Kryptofix and residual 18F are also retained on the cartridge, while the purified radiotracer is obtained after elution: AFFINIMIP® SPE 18F – Aromatic Nucleophilic Substitution
- Purification of 18FBA (Fluorobenzaldehyde) : AFFINIMIP® SPE 18F – Aromatic Nucleophilic Substitution
- Purification of 18F-EFB (Ethyl 4-Fluorobenzoate) : AFFINIMIP® SPE 18F – Aromatic Nucleophilic Substitution
- Removal or concentration of anions : AttractSPETM WAX/SAX
- Removal or concentration of cations : AttractSPETM WCX/SCX
- Separation of hydrophobic/hydrophilic components (18F-product/salts): AttractSPETM HLB or DVB
- Rapid and simple radiotracer purification: no need for a preparative HPLC thanks to our SPE cartridges
- Increased reliability of radiotracer production: reduction of manual handling steps
- Improved radiotracer purity: no cross contamination thanks to single use cartridges
- Compatible with radiofluoration automates
- Radiotracers purification
Fluorobenzoate using AFFINIMIP® SPE 18F - Aromatic Nucleophilic Substitution
- Radiotracers purification
Fluorobenzaldehyde (FBA) using AFFINIMIP® SPE 18F - Aromatic Nucleophilic Substitution
- Development & automation of a nove l[18F]F prosthetic group, 2-[18F]-fluoro-3-pyridinecarboxaldehyde, and its application to an amino(oxy)-functionalised Aβ peptide, O. Morris, J. Gregory, M. Kadirvel, F. Henderson, A. Blykers, A. Mc Mahon, M. Taylor, D. Allsop, S. Allan, J. Grigg, H. Boutin, C. Prenant, Applied RadiationandIsotopes, 116 (2016) 120-127. (Open access article) NEW!
In this article, the authors describe the automated synthesis of [18F]FPCA peptide, a novel, water-soluble prosthetic group using AFFINIMIP®SPE 18F – Aromatic Nucleophilic Substitution purification. It’s radiochemistry has been developed and fully-automated for application in chemoselective radiolabelling of amino(oxy)-derivatised RI-OR2-TAT peptide, (Aoa-k)-RI-OR2-TAT, using a GE TRACERlab FX-FN. RI-OR2-TAT is a brain-penetrant, retro-inverso peptide that binds to amyloid species associated with Alzheimer’s Disease.
- PET Imaging of Macrophage Mannose Receptor-Expressing Macrophages in Tumor Stroma Using 18F-Radiolabeled Camelid Single-Domain Antibody Fragments, A. Blykers, S. Schoonooghe, C. Xavier, K. D’hoe, D. Laoui, M. D’Huyvetter, I. Vaneycken, F. Cleeren, G. Bormans, J. Heemskerk, G. Raes, P. De Baetselier, T. Lahoutte, N. Devoogdt, J. A. Van Ginderachter, and V. Caveliers, J Nucl Med, 2015; 56:1265-1271 DOI: 10.2967/jnumed.115.156828 (Open access article) NEW!
The prosthetic group N-succinimidyl-4-18F-fluorobenzoate (18F-SFB) was purified with AFFINIMIP®SPE 18F – Aromatic Nucleophilic Substitution.
- Preclinical validations of [18F]FPyPEGCBTc(RGDfK): a 18F-labelled RGD peptide prepared by ligation of 2-cyanobenzothiazole and 1,2-aminothiol to image angiogenesis, D. J. Colin, J. A. H. Inkster, S. Germain and Y. Seimbille,EJNMMI Radiopharmacy and Chemistry, 2016, 1:16 DOI: 10.1186/s41181-016-0019-z (Open access article) NEW!
- A novel 2-cyanobenzothiazole-based 18F prosthetic group for conjugation to 1,2-aminothiol-bearing targeting vectors J. A. H. Inkster, D. J. Colin and Y. Seimbille, Org. Biomol. Chem., 2015,13, 3667-3676 DOI: 10.1039/C4OB02637C
In this article, the authors describe the preparative synthesis of [18F]FPyPEGCBT for which its HPLC purification was replaced by AFFINIMIP®SPE 18F – Aromatic Nucleophilic Substitution purification with a DMF elution of the product of interest. The authors conclude that the advantage of such purification are “the obviation of a time-consuming HPLC step and a significant simplication of the overall radiopharmaceutical protocol.”
- 18F-Anti-MMR-nanobodies for PET imaging of tumor-associated macrophage subtypes as surrogate markers for tumor hypoxia, Anneleen Blykers, Catarina Xavier, Ilse Vaneycken, Damya Laoui, Nick Devoogdt, Matthias D’huyvetter, Tony Lahoutte, Jo A. Van Ginderachter and Vicky Caveliers, European Molecular Imaging Meeting (EMIM), Turin, Italy, 26-28 May 2013. In this work, N-succinimidyl-4-18F-fluorobenzoate (18F–SFB) was successfully purified after synthesis with AFFINIMIP®SPE 18F – Aromatic Nucleophilic Substitution.
- Rapid Purification of Fluorine-18 Containing Synthons Using Molecularly Imprinted Polymer Cartridges, G. Smith, S. Bayoudh, C. Pérollier, R. Bhalla, International Symposium on Radiopharmaceutical Sciences (ISRS), Jeju, Korea, 12-17 May 2013. Works carried out in collaboration with GE Healthcare.
- Fast and Selective Purification of a Fluorous Radiotracer using SPE based on Molecularly Imprinted Polymers, C. Pérollier, F. Alix, S. Bayoudh, K. Naraghi, European Association of Nuclear Medicine (EANM), Milan, Italy, October 27-31, 2012.