Peptide fractionation

BioSPE® for peptide fractionation (PepFrac) is used by hydrophobicity at basic pH. This fractionation method being orthogonal to peptide separation by reversed-phase liquid chromatography, sample complexity is reduced for deep proteome sequencing and quantitative analysis.

The BioSPE® PepFrac kit is composed of:

• Fractionation columns based on an innovative reversed-phase resin
• Adapters (for tips centrifugation) and/or collection tools (centrifuge tubes or collection plates). Each kit contains nine collection tools (one for the conditioning/loading/washing steps and one for each elution fraction)
• A detailed protocol for fast and efficient peptide fractionation (up to 8 fractions)
• Triethylamine (TEA) solution

To determine which format and capacity will best suit your needs, please refer to the tables in the “Formats & Capacities” tab.

• Increased number of identified proteins compared to unfractionated samples (up to 30% increase)
• Minimized fraction overlapping and good distribution of peptides in all fractions
• No desalting step required prior to LC-MS/MS analysis (contrary to fractionation by salt)
• No storage constraint (store dry at room temperature)
• Different formats available to adapt to sample volume and throughput (StageTips, Spin columns, 96 wellplates and SPE cartridges)
• Two capacities available for each format depending on your peptide amount

Details regarding the loading and elution volumes for our different BioSPE® for peptide fractionation (PepFrac) products to find the best product, depending on your sample volume.

Information table regarding the capacities (maximal amount of peptides/proteins that can be retained) of our different BioSPE® for peptide fractionation (PepFrac) products :

• For Standard capacity, product references contain the letter S (e.g. Tips-PepFrac.S8.200.96).
• For High capacity, product references contain the letter H (e.g. Tips-PepFrac.H8.200.96)