AFFINIMIP® SPE Deoxynivalenol

Specific extraction of Deoxynivalenol (DON)

Deoxynivalenol (DON) also known as Vomitoxin is a type B trichothecene produced by various Fusarium fungi and occurs predominantly in grains such as wheat, barley, oats, rye, and maize. Although DON is one of the least acutely toxic trichothecenes, it should be treated as an important food safety issue because it is a very common contaminant of grain.

The U.S. Food and Drug Administration has established a level of 1 ppm (parts per million) restriction of vomitoxin.In Europe, Regulation (EC) N°1126/2007 sets maximum levels for Deoxynivalenol in cereals with, for instance, respectively 1750µg/kg for unprocessed maize, 750µg/Kg for cereal flours and 200µg/kg for babyfood.

AFFINIMIP® SPE Deoxynivalenol are selective solid-phase extraction cartridges for the extraction of Deoxynivalenol (DON) and its analogues 3-AcetylDON and 15-AcetylDON prior to analysis by HPLC from cereals such as Oat, Wheat, Corn or processed food (pasta...) and babyfood.

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Benefits

Extraction of Deoxynivalenol, 3-AcetylDON and 15-AcetylDON
High recovery yield (>80%)
Efficient clean-up on cereals
Ready to use and robust protocols
2 extraction protocols of the mycotoxin from cereals: Hydro-organic extraction or Water extraction
Storage at RT over a very long period

Main available formats

Designation, Format of cartridges, amount of sorbent	Box composition	Reference
AFFINIMIP® SPE Deoxynivalenol - 6mL/100mg cartridge for food and babyfood
	50 cartridges	FS117-03B
AFFINIMIP® SPE Deoxynivalenol - 6mL/200mg cartridge for animal feed
	50 cartridges	FS117-03B-200mg
Standard solution Deoxynivalenol - 100µg/mL	1mL vial	REA-DON-1mL
Standard solution 3-AcetylDeoxynivalenol - 100µg/mL	1mL vial	REA-3AcDON-1mL
Standard solution 15-AcetylDeoxynivalenol - 100µg/mL	1mL vial	REA-15AcDON-1mL

Specific formats for automates

AFFINIMIP®SPE Deoxynivalenol can be used with automates with the appropriate reference.

Designation, Format of cartridges	Box composition	Reference
AFFINIMIP® SPE Deoxynivalenol for Multipurpose sampler automate (GERSTEL)-6mL/100mg cartridge for food and babyfood	50 cartridges	FS117-03B-GERSTEL
AFFINIMIP® SPE Deoxynivalenol for Multipurpose sampler automate (GERSTEL)-6mL/200mg cartridge for animal feed	50 cartridges	FS117-03B-200mg-GERSTEL
AFFINIMIP® SPE Deoxynivalenol for ASPEC automate (GILSON)-6mL/100mg cartridge for food and babyfood	50 cartridges	FS117-03B
AFFINIMIP® SPE Deoxynivalenol for ASPEC automate (GILSON)-6mL/200mg cartridge for animal feed	50 cartridges	FS117-03B-200mg

Other formats can be supplied on demand

Application notes

Analysis of Deoxynivalenol in Meat New!

The extraction of Deoxynivalenol on Meat was carried out with AFFINIMIP® SPE Deoxynivalenol before analysis.

Application note AFFINIMIP® SPE Deoxynivalenol - Meat

Cereals with two extraction methods of Deoxynivalenol: hydro-organic or Water

The extraction of Deoxynivalenol on Cereals was carried out with two different protocols: Water and Hydro-organic (a mixture of Acetonitrile and Water). Then, the extracts were used for a clean-up with AFFINIMIP® SPE Deoxynivalenol before analysis.

Application note AFFINIMIP® SPE Deoxynivalenol - Cereals with two extration methods

Deoxynivalenol and its analogues 3-AcetylDON and 15-AcetylDON in cereals

The extraction of Deoxynivalenol and its analogues on Cereals was carried out with AFFINIMIP® SPE Deoxynivalenol before analysis by a single MS quadrupole.

Application note AFFINIMIP® SPE Deoxynivalenol - DON and 3-AcDON and 15-AcDON

Analysis of Deoxynivalenol in babyfood

The extraction of Deoxynivalenol in babyfood was carried out with AFFINIMIP® SPE Deoxynivalenol before analysis by a single MS quadrupole.

Application note AFFINIMIP® SPE Deoxynivalenol - DON in babyfood

Analysis of Deoxynivalenol in animal feed

Several animal feeds were analyzed by UV after a clean-up with AFFINIMIP® SPE Deoxynivalenol.

Application note AFFINIMIP® SPE Deoxynivalenol - DON in animal feed