AttractSPE®Disks BioMol

As protein analysis has become more complex and more sensitive, the need for adequate materials meets more specific requirements. Proteomic sample preparation should be a critical consideration.

AFFINISEP brings to you a full range of membranes with AttractSPE®Disks BioMol with C18, C8, C4, SCX, SAX, RPS….

These SPE membranes are especially designed and made by AFFINISEP for your bioanalytical needs and to help you to develop your own tools for your R&D projects, for instance to make Stagetips by punching the membranes. Our high quality SPE membranes allow high throughput (HTS) for protein desalting or peptides purification.

AttractSPE®Disks BioMol are fully compatible with automated liquid handlers. Indeed, with these Solid Phase Extraction Disks, you can design the applications you wish such as removing impurities from samples and isolation of analytes from biomatrices with small elution volumes. Fast and efficient purification of peptides through Solid Phase Extraction is easily accessible.

• For peptides/proteins/DNA & RNA purification and desalting or small molecules extraction
• Diversity of sorbents C18, C8, C4, SCX, SAX, RPS
• Easy adaptation to Bioanalytical formats
• Handling of tiny and viscous samples
• High density of homogeneous particles
• Compatible with automated liquid handlers and high throughput analysis (HTS)
• High capacity
• Several biotools are available using these SPE disks (Stage-tips, Spin columns, 96 well plate for microelution, cartridges)

• The HUSH complex represses retroviruses, transposons and genes to maintain the integrity of vertebrate genomes. In this paper, it was demonstrated that TASOR, the central HUSH subunit, is a multifunctional pseudo-PARP that directs HUSH assembly and epigenetic regulation of repetitive genomic targets.Clean-up of tryptic digests of proteins extracted from TASOR cells was performed with AttractSPE® Disks Bio RPS (SPE-Disks-Bio-RPS-M-47.20) packed into 200 µL pipette tips (equivalent to our AttractSPE® Disks Tips RPS, ref Tips-RPS-M.T1.200.96) prior to mass spectrometry analysis.TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control, Christopher H. Douse, Iva A. Tchasovnikarova, Richard T. Timms, Anna V. Protasio, Marta Seczynska, Daniil M. Prigozhin, Anna Albecka, Jane Wagstaff, James C. Williamson, Stefan M. V. Freund, Paul J. Lehner, Yorgo Modis, Nature Communications, 2020, https://doi.org/10.1038/s41467-020-18761-6.

• Clathrin-mediated endocytosis (CME) is a central pathway for the internalization of proteins from the cell surface, thereby contributing to the maintenance of the plasma membrane protein composition. In this article, it was hypothesized that blocking AP-2 function, a key component for the formation of endocytic clathrin-coated vesicles (CCVs), and thus inhibiting CME may be a novel approach to identify new druggable targets, or to increase their residence time at the plasma membrane, thereby increasing the probability for efficient therapeutic intervention. Stage tips packed with two layers of AttractSPE® Disks Bio RPS (SPE-Disks-Bio-RPS-M-47.20) and equivalent to our AttractSPE® Disks Tips RPS (Tips-RPS-M.T2.200.96) were used for peptides desalting after tryptic digestion.Inhibition of clathrin-mediated endocytosis by knockdown of AP-2 leads to alterations in the plasma membrane proteome, David Tobys, Lisa Maria Kowalski, Eva Cziudaj, Stefan Müller, Peter Zentis, Elke Pach, Paola Zigrino, Tobias Blaeske, Stefan Höning, Traffic, Volume 22, Issue 1-2, 2021, https://doi.org/10.1111/tra.12770.