New posters on proteomics!

For peptide desalting

For peptide fractionation

AttractSPE®Disks Tips C18 – Desalting

  • Microelution SPE Tips made with AttractSPE® Disks C18 sorbent: soft, thin and mechanically stable membrane, made of small sorbent particles tightly embedded, with reduced dead volume, small elution volume and high capacity
  • More than 97% proteins identified after desalting of HeLa digest on AttractSPE®Disks Tips C18 (200µL), with no loss of peptides
  • Good retention of peptides on the whole polarity range
  • Excellent repeatability (RSD < 3%)
  • Broad range of use: from single cell-like analysis (1ng) to high peptide amounts (> 10 µg)

BioSPE PepFrac – Fractionation

  • New reversed-phase resin based on Affinisep disks technology for peptide fractionation at high pH (orthogonal to RPLC peptide separation): reduction of sample complexity for deep proteome sequencing and quantitative analysis
  • Fractionation of 50µg peptides resulting from the tryptic digestion of proteins contained in HEK cells: 8 fractions with ACN gradient (2% to 50%) on BioSPETM PepFrac 200µL Tips
  • High number of proteins identified (increase of 20% compared to unfractionated samples)
  • Good distribution of peptides in all fractions and efficient fractionation with minimum fraction overlapping
  • Results similar to competitor fractionation kit but no storage constraints for BioSPETM PepFrac (store dry at room temperature) and evaporation time divided by two
  • Flexibility of format and capacity: BioSPETM PepFrac available as spin columns for high peptide amounts and as 96 wellplates for high throughput experiments

Do not hesitate to contact us for any further information!

posters proteomics vignette

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