Aminoglycosides
Aminoglycosides are an important class of broad-spectrum antibiotics frequently used to treat bacterial infections such as some Gram -positive and mainly gram-negative bacteria. However, their use is limited due to side effects of nephrotoxicity and ototoxicity and due to antimicrobial resistance to some of these molecules.
Aminoglycosides are still frequently used for veterinary use to treat infections in cattle, pigs, sheep, goat or horses.
Nevertheless, food safety authorities have established maximum residue limits (MRLs) in several countries in Europe, China, USA, Canada or by the codex alimentarius of FAO/WHO. These MRLs are defined for a combination of molecules/matrices.
Affinisep has developed a ready-to-use SPE kit AFFINIMIP®SPE Aminoglycosides for the analysis of these residues in food matrices.
This publication shows the results of 5 independent laboratories applying the official AOAC First Action Method 2020.04 developed by Société des Produits Nestlé for the screening of 152 veterinary drug residues by LC-MS/MS in a wide variety of food commodities such as milk-based ingredients, meat/fish-based food ingredients, eggs and animal byproducts and fats.
To screen these drugs, 4 streams were developed. A stream was specifically implemented for 14 aminoglycosides using Molecularly Imprinted Polymers SPE columns such as AFFINIMIP®SPE Aminoglycosides. AOAC has approved the method for Final Action status thanks to these results.
Aurélien Desmarchelier, Thomas Bessaire, Marie-Claude Savoy, Adrienne Tarres, Claudia Mujahid, Andrea Beck, Pascal Mottier, Xun Fu, Wai-Chinn Chan, Stéphanie Laborie, Nadine Hagenbourger, Gislaine Miranda, Thierry Delatour, Screening of 152 Veterinary Drug Residues in Animal Source Foods by LC–MS/MS, Multilaboratory Validation Study: Final Action 2020.04, Journal of AOAC INTERNATIONAL, Volume 107, Issue 4, July-August 2024, Pages 617–631.