Estrogens, Estradiol, Ethinylestradiol
Estrogens are a group of compounds which play an important role in the estrous cycle. They are either natural (Estrone, Estriol, 17α- and 17β-Estradiol) or synthetic compounds (17α-EthinylEstradiol, Dienestrol, Diethylstilbestrol).
Estrogens play a key role in developmental and reproductive functions. They also affect a diversity of biological processes involved in coronary artery disease, immunocompetence and cancer susceptibility. When they are present in wastewater, these endocrine disrupting chemicals (EDC) have adverse effects on endocrine systems of human beings and animals.
To facilitate analyses, Affinisep has developed AFFINIMIP® SPE Estrogens, selective solid phase extraction cartridges that selectively clean and concentrate estrogens before analysis by HPLC.
Our Booklet AFFINIMIP®SPE Estrogens collects main useful information about :
- Regulations concerning Estrogens
- AFFINIMIP®SPE Estrogens description
- Product added-value vs alternative methods
- Application notes
This article compares the extraction of steroidal estrogens from tap water by using two extractions method, Liquid – Liquid Extraction and SPE with AFFINIMIP®SPE Estrogens prior analysis with GC-MS/MS and GC-HRMS. For SPE, recovery yields between 99 and 113% were obtained.
Zacs, D., Perkons, I. and Bartkevics, V., (2016) ‘Determination of steroidal oestrogens in tap water samples using solid-phase extraction on a molecularly imprinted polymer sorbent and quantification with gas chromatography-mass spectrometry (GC-MS)‘. Environmental Monitoring and Assessment, 188, p.433.
Please note the article using AFFINIMIP®SPE Phenolics for the determination of ultra trace levels of 13 estrogenic EDCs by UHPLC-MS/MS
13 analytes: estrone (E1), 17α-estradiol (α-E2), 17β-estradiol (β-E2), estriol (E3), 17α-ethinylestradiol (EE2), diethylstilbestrol (DES), bisphenol A (BPA), bisphenol S (BPS), 4-n-octylphenol (OP), 4-n-nonylphenol (NP), coumestrol (COU), genistein (GEN), and enterolactone (ENT).
Bousoumah, R., Antignac, J.P., Camel, V., Grimaldi, M., Balaguer, P., Courant, F., Bichon, E., Morvan, M.-L. and Le Bizec, B, (2015) ‘Development of a molecular recognition based approach for multi-residue extraction of estrogenic endocrine disruptors from biological fluids coupled to liquid chromatography-tandem mass spectrometry measurement’. Analytical and Bioanalytical Chemistry, 407(30), pp.8713–8723.
Mercurio, S., Tremolada, P., Nobile, M., Fernandes, D., Porte, C. and Sugni, M., (2015) ‘Unravelling estradiol metabolism and involvement in the reproductive cycle of non-vertebrate animals: The sea urchin model‘. Steroids, 104, pp.25–36.
Matějíček, D., Burešová, A., Vlček, J. and Pelcová, P., (2013) ‘On-line molecularly imprinted solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry for the determination of hormones in water and sediment samples‘. Journal of Separation Science, 36(9–10), pp.1509–1515.
Matějíček, D., Grycová, A. and Vlček, J., (2013) ‘The use of molecularly imprinted polymers for the multicomponent determination of endocrine-disrupting compounds in water and sediment‘. Journal of Separation Science, 36(6), pp.1097–1103.
Doué, M., Bichon, E., Dervilly-Pinel, G., Pichon, V., Chapuis-Hugon, F., Lesellier, E., West, C., Monteau, F. and Le Bizec, B., (2012) ‘Molecularly imprinted polymer applied to the selective isolation of urinary steroid hormones: An efficient tool in the control of natural steroid hormones abuse in cattle‘. Journal of Chromatography A, 1270, pp.51–56.
Lucci, P., Núñez, O. and Galceran, M.T., (2011) ‘Solid-phase extraction using molecularly imprinted polymer for selective extraction of natural and synthetic estrogens from aqueous samples‘. Journal of Chromatography A, 1218(30), pp.4828–4833.
This poster was presented at Euroresidues VIIth conference, Egmond aan Zee, The Nertherlands, 14th-16th May 2012 by the French reference laboratory on residues and contaminants in food (LABERCA – ONIRIS).
S. Rochereau, E. Bichon, F. Courant, F. Monteau, S. Prévost, F. Hanganu, N. Cesbron, G. Dervilly-Pinel, B. Le Bizec, (2012) ‘Quantification of estrogens at ppt levels in bovine plasma by AFFINIMIP® SPE and GC-MS/MS analysis’
Poster presented at ISEAC-37 Antwerp, Belgium, May 22nd, 2012.
D.Derrien, M. Mulet, B. Chevalier, F. Alix, C.Pérollier, O.Lépine, K.Naraghi* , Johann Travers, S. Bayoudh, (2012) ‘Original method for analysis of Estrogens and Bisphenol A, Endocrine Disrupting Chemicals using solid phase extraction based on molecularly imprinted polymer’
Poster presented at the 2nd International Symposium on HTSP , Brugge, Belgium, 31st January – 3rd February 2012 by the French reference laboratory on residues and contaminants in food (LABERCA – ONIRIS).
Doué, M., Bichon, E., Monteau, F. and Le Bizec, B., n.d. ‘High efficiency of semi-preparative supercritical fluid chromatography with molecularly imprinted polymer as stationary phase (SFC-MIP): Application on urinary steroids purification for IRMS analysis‘
This conference was presented at 5th international Symposium on Recent Advances in Food Analysis (RAFA), Prague, Czech Republic, 1-4 November 2011 by the French reference laboratory on residues and contaminants in food (LABERCA – ONIRIS).
E. Bichon, S. Rochereau, L. Sérée, S. Prevost, F. Monteau, B. Le Bizec. ‘How to improve analytical strategies to monitor growth promoting agents misuse in cattle’
Poster presented at PITTCOIN, Atlanta, Georgia, 13-18 March 2011
Derrien, D., Pérollier, C., Lépine, O., Bayoudh, S. and Naraghi, K., (2011) ‘New method for extraction of estrogens on molecularly imprinted polymer‘.
