Patulin

Patulin is a mycotoxin produced by a variety of molds, particularly Aspergillus and Penicillium. It is commonly found in rotting apples, and the amount of patulin in apple products is generally viewed as a measure of the quality of the apples used in production.

Studies have shown that it is genotoxic. Several countries have instituted patulin restrictions in apple products. The World Health Organization, the FDA and the European Union recommend a maximum concentration of 50µg/L in apple juice and 25µk/kg in apple puree. Member countries of the European Union have set maximum allowable levels of patulin at 10μg/kg in apple juice and solid apple products, including apple compote and apple puree, for infants and young children (European Commission Regulation (EC) 1881/2006).

Here you can find several application notes for the analysis of Patulin in various matrix based on AFFINIMIP® SPE Patulin kits.

Our Booklet AFFINIMIP®SPE Patulin collects main useful information about :

  • Regulations concerning Patulin
  • AFFINIMIP®SPE Patulin description
  • Proficiency testing obtained with this product
  • Product added-value vs alternative methods
  • Application notes
  • Relative products (standard solution…)

> See our Booklet AFFINIMIP® SPE Patulin.

Pear & apple puree as well as apple juice protocols were developed.

  • Mycotoxins / 
  • Patulin

AFFINIMIP SPE Patulin Apple juice LC - MS/MS

New ! Combination of AFFINIMIP® SPE PATULIN clean-up with LC-MS/MS detection strongly shorten Patulin analysis with excellent recoveries and repeatability!

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  • Mycotoxins / 
  • Patulin

Application note AFFINIMIP®SPE Patulin - Apple juice and apple puree tested on a HPLC system

Apple juice and apple puree spiked with Patulin were tested on a HPLC system after sample cleanup with AFFINIMIP®SPE Patulin. The chromatograms obtained show that the main matrix interferent, 5-Hydroxymethylfurfural (HMF) is removed. Average Recoveries of 90.6% were obtained in apple juice spiked at 40μg/kg and 84% in apple puree spiked at 20μg/kg. Recoveries > 80% were also obtained in baby food at maximum allowable level for infants and young children (10μg/kg).

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  • Mycotoxins / 
  • Patulin

Application note AFFINIMIP® SPE Patulin in Thick juices and fruit juice concentrate

Thick juices and fruit juice concentrates analysis with AFFINIMIP®SPE Patulin by UV detection
Patulin could be analyzed on thick juices and fruit juice concentrates with recovery yields higher than 80% after a pectinase treatment and an efficient and fast clean-up with AFFINIMIP®SPE Patulin.

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  • Mycotoxins / 
  • Patulin

Application note AFFINIMIP® SPE Patulin - Analysis in apple juice & apple puree in baby food – UV detection

Recoveries > 80% were obtained in baby food at maximum allowable level for infants and young children (10μg/kg).

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  • Mycotoxins / 
  • Patulin

Application note AFFINIMIP®SPE Patulin in cider

Cider – UV detection
Cider spiked with Patulin at 10µg/kg and 40 µg/kg was tested on a HPLC system after sample cleanup with AFFINIMIP® SPE Patulin. Clean chromatograms and recoveries above 80% were obtained.

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  • Mycotoxins / 
  • Patulin

Application note AFFINIMIP®SPE Patulin in dried apple

Dried apple analysis with AFFINIMIP®SPE Patulin 6mL-200mg – UV detection
dried apple dices spiked with Patulin at 10µg/kg and 20 µg/kg were tested on a HPLC system after sample cleanup with AFFINIMIP®SPE Patulin 200mg cartridges. Clean extracts and recoveries above 90% were obtained.

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  • Mycotoxins / 
  • Patulin

Application note AFFINIMIP®SPE Patulin automated protocol

Automated protocol for Patulin analysis in apple juice – UV detection by AFFINIMIP® SPE Patulin
The combination of AFFINIMIP®SPE Patulin cartridges with an automate GILSON GX-271 ASPEC™ shows accurate and reliable analysis with recovery yields higher than 80% on a apple juice.

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  • Mycotoxins / 
  • Patulin

Application note AFFINIMIP® SPE Patulin apple-fruit puree

Patulin analysis in Apple-fruit puree – UV detection by AFFINIMIP® SPE Patulin
Apple/pear and apple/strawberry purees were tested on a HPLC system after sample cleanup with AFFINIMIP®SPE Patulin. Recoveries above 75% were obtained.

Cider analysis by UV detection using AFFINIMIP® SPE PATULIN
Cider spiked with Patulin at 10µg/kg and 40 µg/kg was tested on a HPLC system after sample cleanup with AFFINIMIP® SPE Patulin. Clean chromatograms and recoveries above 80% were obtained.

DownloadPDF - 589.13kB