Ochratoxin A
Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite of various Aspergillus and Penicillium fungis. It can be found on several commodities (e.g. cereals and cereal-based products, coffee, beer, grape juice, wine, cacao products).
Ochratoxin A exhibits toxicity in animals and mankind, including nephrotoxic, hepatotoxic, immunotoxic, teratogenic and carcinogenic effects and represents therefore a serious health risk to livestock and population. In order to limit these effects, European Regulation (EC) 1881/2006 sets maximum levels for Ochratoxin A in foodstuffs (e.g. 5μg/kg in raw cereal grains, 30μg/kg in spices).
You can find here several application notes as well as articles using AFFINIMIP® SPE Ochratoxin A in various matrix such as cereals, wines, … as well the automatisation of its analysis. Flangeless cartridges are adapted to the main automate on the market.
- Mycotoxins /
- Ochratoxin A
Application note AFFINIMIP® SPE Ochratoxin A Automated Protocol (GILSON GX271 ASPEC™)
Automated protocol for Analysis of Ochratoxin A
The combination of AFFINIMIP® SPE Ochratoxin A SPE cartridges with an automate GILSON GX-271 ASPEC™ shows accurate and reliable analysis (CV 1.7% vs CV 2.5% in manual mode).
- Mycotoxins /
- Ochratoxin A
Application note AFFINIMIP® SPE Ochratoxin A Red and White Wines
Analysis of Ochratoxin A Red & white wines
Ochratoxin A concentrations in red and white wines were determined after pre-treatment with AFFINIMIP® SPE Ochratoxin A. High recovery yields were obtained via a very simple protocol.
- Mycotoxins /
- Ochratoxin A
Application note AFFINIMIP® SPE Ochratoxin A Cereals and Spices
Analysis of Ochratoxin A in Cereals and spices
Ochratoxin A analysis has been carried out on Wheat and several spices (Paprika, grey Pepper). After cleanup and concentration with AFFINIMIP® SPE Ochratoxin A, recovery yields higher than 90% (concentration: 30µg/Kg) were obtained except for Grey Pepper (73% for a concentration of 5µg/Kg).
In this article, a method of analysis Ochratoxin A in edible tissues was developed and validated in pig complex matrix. With this very simple method, very sensitive LOD and LOQ respectively 0.001 and 0.003 μg/kg were obtained thanks to a cleanup with AFFINIMIP®SPE Ochratoxin A.
Luci, G., (2020) ‘A rapid HPLC-FLD method for Ochratoxin A detection in pig muscle, kidney, liver by using enzymatic digestion with MISPE extraction‘. MethodsX, 7, p.100873. Open access
Mishra, R.K., Hayat, A., Catanante, G., Istamboulie, G. and Marty, J.-L., (2016) ‘Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor‘. Food Chemistry, 192, pp.799–804.
Andrade, M.A. and Lanças, F.M., (2015) ‘Estado-da-arte na análise cromatográfica de Ocratoxina A em amostras de alimento‘. Scientia Chromatographica’, 7(1), pp.31–52 Open access
(In portuguese)
Xie, L., Sheng, P., Kong, W., Zhao, X., Ou-Yang, Z. and Yang, M., (2015) ‘Solid-phase extraction using molecularly imprinted polymer for determination of ochratoxin A in human urine’, World Mycotoxin Journal, 8(1), pp.37–44.
Ibarz, R., Garvín, A., Azuara, E. and Ibarz, A., (2015) ‘Modelling of ochratoxin A photo-degradation by a UV multi-wavelength emitting lamp‘. LWT – Food Science and Technology, 61, pp.385–392.
Cao, J., Zhou, S., Kong, W., Yang, M., Wan, L. and Yang, S., (2013) ‘Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation’. Food Control, 33(2), pp.337–343.
Cao, J., Kong, W., Zhou, S., Yin, L., Wan, L. and Yang, M., (2013) ‘Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine, and grape juice’. Journal of Separation Science, 36(7), pp.1291–1297.
Lee, T.P., Saad, B., Salleh, B. and Mat, I., (2013) ‘Micro-solid phase extraction of ochratoxin A, and its determination in urine using capillary electrophoresis‘. Microchimica Acta, 180, pp.1149–1156.
Lee, T.P., Saad, B., Khayoon, W.S. and Salleh, B., (2012) ‘Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine‘. Talanta, 88, pp.129–135.
Hadj Ali, W., Derrien, D., Alix, F., Pérollier, C., Lépine, O., Bayoudh, S., Chapuis-Hugon, F. and Pichon, V., (2010) ‘Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals‘. Journal of Chromatography A, 1217, pp.6668–6673.
| Product Image | Product Name | Product Description | Product Price | Cart Actions | |
|---|---|---|---|---|---|
|
SignIn To View | SignIn To View | |||
|
SPE Vacuum Manifold 24-port
Ref. : ACC-MAN24 |
SignIn To View | SignIn To View | ||
|
12-port model
Ref. : ACC-MAN1 |
SignIn To View | SignIn To View | ||
|
SignIn To View | SignIn To View | |||
|
SPE Vacuum Manifold – 12-port model
Ref. : ACC-MAN2 |
1 unit |
SignIn To View | SignIn To View | |
|
|
1L trap kit
Ref. : ACC-TRAP |
SignIn To View | SignIn To View | ||
|
SignIn To View | SignIn To View | |||
|
AFFINIMIP® SPE Ochratoxin A
Ref. : FS101-03 |
Selective SPE cartridges for OTA - 50/pk - 3mL |
SignIn To View | SignIn To View | |
 |
AFFINIMIP® SPE Ochratoxin A
Ref. : FS101-04TL |
Selective SPE cartridges for OTA - 100 /pk - 3mL flangeless |
SignIn To View | SignIn To View | |
|
AFFINIMIP® SPE Ochratoxin A
Ref. : FS101-03B |
Selective SPE cartridges for OTA - 50/pk - 6mL |
SignIn To View | SignIn To View | |
|
|
AFFINIMIP® SPE Ochratoxin A
Ref. : FS101-04BTL |
Selective SPE cartridges for OTA - 100/pk - 6mL flangeless |
SignIn To View | SignIn To View |